Application | Comment | Organism |
---|---|---|
biotechnology | recombination of the catalytic domains of three glycoside hydrolase family 48 bacterial cellulases (Cel48), i.e. Clostridium cellulolyticum CelF, Clostridium stercorarium CelY, and Clostridium thermocellum CelS, to create a diverse library of Cel48 enzymes with an average of 106 mutations from the closest native enzyme. The library is based on the Clostridium thermocellum CelS architecture, which consists of a 70-kDa catalytic domain connected to the organism's respective dockerin domain. Large variations in properties such as the functional temperature range, stability, and specific activity on crystalline cellulose are found. Functional status and stability are predictable from simple linear models of the sequence-property data. Recombined protein fragments contribute additively to these properties in a given chimera | Thermoclostridium stercorarium |
biotechnology | recombination of the catalytic domains of three glycoside hydrolase family 48 bacterial cellulases (Cel48), i.e. Clostridium cellulolyticum CelF, Clostridium stercorarium CelY, and Clostridium thermocellum CelS, to create a diverse library of Cel48 enzymes with an average of 106 mutations from the closest native enzyme. The library is based on the Clostridium thermocellum CelS architecture, which consists of a 70-kDa catalytic domain connected to the organism's respective dockerin domain. Two of the most stabilizing blocks are predicted to be from the parent CelS at blocks located in the C-terminus of the catalytic domain, close to where the dockerin attaches. Two of the highly stable chimeras also hydrolyze more cellulose than the most active parental enzyme | Acetivibrio thermocellus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Acetivibrio thermocellus |
expression in Escherichia coli | Thermoclostridium stercorarium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | A3DH67 | - |
- |
Acetivibrio thermocellus DSM 1237 | A3DH67 | - |
- |
Thermoclostridium stercorarium | P50900 | - |
- |
Thermoclostridium stercorarium DSM 8532 | P50900 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
celS | - |
Acetivibrio thermocellus |
CelY | - |
Thermoclostridium stercorarium |