Literature summary for 3.2.1.176 extracted from

Kostylev, M.; Alahuhta, M.; Chen, M.; Brunecky, R.; Himmel, M.E.; Lunin, V.V.; Brady, J.; Wilson, D.B.
Cel48A from Thermobifida fusca: structure and site directed mutagenesis of key residues (2014), Biotechnol. Bioeng., 111, 664-673.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
mutant D224N, to 1.6 A resolution. There is one molecule in the asymmetric unit in complex with a cellobiose and a cellohexaose molecule. The active site tunnel entrance is enriched in aromatic residues. Only the aromatic residues located around the tunnel entrance appear to be important for the ability of Cel48A to access individual cellulose chains on bacterial cellulose	Thermobifida fusca

Protein Variants

Protein Variants	Comment	Organism
F195A	mutagenesis of active site tunnel residues, moderate reduction in activity	Thermobifida fusca
W313A	residue is important for processivity on bacterial cellulose	Thermobifida fusca
W315A	residue is important for processivity on bacterial cellulose	Thermobifida fusca
Y213A	mutagenesis of active site tunnel residues, moderate reduction in activity	Thermobifida fusca
Y213A/S311A	mutagenesis of active site tunnel residues, moderate reduction in activity	Thermobifida fusca
Y213A/W313A	mutagenesis of active site tunnel residues, moderate reduction in activity	Thermobifida fusca
Y97A	mutagenesis of active site tunnel residues, moderate reduction in activity	Thermobifida fusca

Organism

Organism	UniProt	Comment	Textmining
Thermobifida fusca	Q47NH7	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
bacterial cellulose + H2O	-	Thermobifida fusca	cellobiose + cellotriose	no release of D-.glucose is observed	?
phosphoric acid-swollen cellulose + H2O	-	Thermobifida fusca	cellobiose + cellotriose	no release of D-.glucose is observed	?

Synonyms

Synonyms	Comment	Organism
Cel48A	-	Thermobifida fusca
Tfu_1959	-	Thermobifida fusca

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Release 2023.1 (January 2023)