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Literature summary for 3.2.1.176 extracted from

  • Hobdey, S.E.; Knott, B.C.; Haddad Momeni, M.; Taylor, L.E.; Borisova, A.S.; Podkaminer, K.K.; VanderWall, T.A.; Himmel, M.E.; Decker, S.R.; Beckham, G.T.; Stahlberg, J.
    Biochemical and structural characterization of two Dictyostelium cellobiohydrolases from the Amoebozoa kingdom reveal a high conservation between distant phylogenetic trees of life (2016), Appl. Environ. Microbiol., 82, 3395-3409.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.176

Crystallization (Commentary)

Crystallization (Comment) Organism
to 1.8 A resolution. Protein natively consists of a catalytic domain and does not exhibit a carbohydrate-binding module. Structure is homologous to those of other GH7 CBHs with an enclosed active-site tunnel Dictyostelium purpureum
to 2.1 A resolution. Protein natively consists of a catalytic domain and does not exhibit a carbohydrate-binding module. Strucutre is homologous to those of other GH7 CBHs with an enclosed active-site tunnel Dictyostelium discoideum

Protein Variants

Protein Variants Comment Organism
additional information protein natively consists of a catalytic domain and does not exhibit a carbohydrate-binding module. Upon addition of the family 1 Hypocrea jecorina Cel7A carbohydrate-binding module and linker to the C terminus of the Dictyostelium enzyme, the recombinant Cel7ACBM hydrolyzes avicel, pretreated corn stover, and phosphoric acid-swollen cellulose as efficiently as Hypocrea jecorina Cel7A when hydrolysis is compared at their temperature optima. The Ki of cellobiose is significantly higher for the Dictyostelium Cel7ACBM Dictyostelium purpureum
additional information protein natively consists of a catalytic domain and does not exhibit a carbohydrate-binding module. Upon addition of the family 1 Hypocrea jecorina Cel7A carbohydrate-binding module and linker to the C terminus of the Dictyostelium enzymes, the recombinant Cel7ACBM hydrolyzes avicel, pretreated corn stover, and phosphoric acid-swollen cellulose as efficiently as Hypocrea jecorina Cel7A when hydrolysis is compared at their temperature optima. The Ki of cellobiose is significantly higher for the Dictyostelium Cel7ACBM Dictyostelium discoideum

Inhibitors

Inhibitors Comment Organism Structure
cellobiose
-
 Dictyostelium discoideum
cellobiose
-
 Dictyostelium purpureum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4
-
 4-nitrophenyl beta-D-lactoside pH 5.0, 45°C, recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium discoideum
3.4
-
 4-nitrophenyl beta-D-lactoside pH 5.0, 45°C, recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium purpureum

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum Q55FE6
-
-
Dictyostelium purpureum F0ZJZ1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-lactoside + H2O
-
 Dictyostelium discoideum 4-nitrophenol + lactose
-
 ?
4-nitrophenyl beta-D-lactoside + H2O
-
 Dictyostelium purpureum 4-nitrophenol + lactose
-
 ?
avicel + H2O
-
 Dictyostelium discoideum ?
-
 ?
avicel + H2O
-
 Dictyostelium purpureum ?
-
 ?
phosphoric acid-swollen cellulose + H2O
-
 Dictyostelium discoideum ?
-
 ?
phosphoric acid-swollen cellulose + H2O
-
 Dictyostelium purpureum ?
-
 ?
pretreated corn stover + H2O
-
 Dictyostelium discoideum ?
-
 ?
pretreated corn stover + H2O
-
 Dictyostelium purpureum ?
-
 ?

Synonyms

Synonyms Comment Organism
Cel7A
-
 Dictyostelium discoideum
Cel7A
-
 Dictyostelium purpureum
DDB0202233
-
 Dictyostelium discoideum
DICPUDRAFT_151874
-
 Dictyostelium purpureum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium discoideum
55
-
 recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium purpureum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
53
-
 melting temperature, recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium discoideum
53
-
 melting temperature, recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium purpureum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.27
-
 4-nitrophenyl beta-D-lactoside pH 5.0, 45°C, recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium discoideum
0.51
-
 4-nitrophenyl beta-D-lactoside pH 5.0, 45°C, recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium purpureum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium discoideum
5
-
 recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium purpureum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.13
-
 cellobiose pH 5.0, 45°C, recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium purpureum
0.295
-
 cellobiose pH 5.0, 45°C, recombinant protein carrying Hypocrea jecorina carbohydrate-binding module and linker Dictyostelium discoideum