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Literature summary for 3.2.1.176 extracted from

  • Jalak, J.; Vaeljamaee, P.
    Multi-mode binding of cellobiohydrolase Cel7A from Trichoderma reesei to cellulose (2014), PLoS ONE, 9, e108181.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.176

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information bacterial cellulose binding at low nanomolar free enzyme concentrations is exclusively active site-mediated and is consistent with Langmuir's one binding site model, strongest binding observed with non-complexed cellulases, productive binding of te enzyme to cellulose chain ends on the hydrophobic face of bacterial cellulose microfibril. With increasing free TrCel7A concentrations the isotherm gradually deviates from the Langmuir's one binding site model caused by the increasing contribution of lower affinity binding modes that included both active site-mediated binding and non-productive binding with active site free from cellulose chain. The binding of enzyme to bacterial cellulose is only partially reversible. Measurement of binding kinetics and modelling, overview Trichoderma reesei

Organism

Organism UniProt Comment Textmining
Trichoderma reesei
-
-
-
Trichoderma reesei QM 9414
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-D-lactoside + H2O
-
 Trichoderma reesei 4-methylumbelliferol + D-lactose
-
 ?
4-methylumbelliferyl beta-D-lactoside + H2O
-
 Trichoderma reesei QM 9414 4-methylumbelliferol + D-lactose
-
 ?
4-nitrophenyl beta-D-lactoside + H2O
-
 Trichoderma reesei 4-nitrophenol + D-lactose
-
 ?
4-nitrophenyl beta-D-lactoside + H2O
-
 Trichoderma reesei QM 9414 4-nitrophenol + D-lactose
-
 ?
bacterial cellulose + H2O binding of enzyme to bacterial cellulose is only partially reversible Trichoderma reesei ?
-
 ?
bacterial cellulose + H2O binding of enzyme to bacterial cellulose is only partially reversible Trichoderma reesei QM 9414 ?
-
 ?

Synonyms

Synonyms Comment Organism
Cel7A
-
 Trichoderma reesei
cellobiohydrolase
-
 Trichoderma reesei

General Information

General Information Comment Organism
additional information bacterial cellulose binding at low nanomolar free enzyme concentrations is exclusively active site-mediated and is consistent with Langmuir's one binding site model, strongest binding observed with non-complexed cellulases, productive binding of te enzyme to cellulose chain ends on the hydrophobic face of bacterial cellulose microfibril. With increasing free TrCel7A concentrations the isotherm gradually deviates from the Langmuir’s one binding site model caused by the increasing contribution of lower affinity binding modes that includes both active site-mediated binding and non-productive binding with active site free from cellulose chain. The binding of enzyme to bacterial cellulose is only partially reversible Trichoderma reesei