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Literature summary for 3.2.1.176 extracted from
- Structural, biochemical, and computational characterization of the glycoside hydrolase family 7 cellobiohydrolase of the tree-killing fungus Heterobasidion irregulare (2013), J. Biol. Chem., 288, 5861-5872.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, apoenzyme using 20 mM MgCl2, 0.1 M HEPES, pH 7.5, 22% (w/v) polyacrylic acid 5100 sodium salt, or in complex with thio-beta-D-xylopentaose using 20mM MgCl2, 0.1 M HEPES, pH 7.7, 15-22% (w/v) polyethylene glycol 3350 | Heterobasidion irregulare |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000, SDS-PAGE | Heterobasidion irregulare |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellulose + H2O | Heterobasidion irregulare | - |
cellobiose + ? | - |
? |  |
| cellulose + H2O | Heterobasidion irregulare TC-32-1 | - |
cellobiose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Heterobasidion irregulare | I1SB08 | - |
- |
| Heterobasidion irregulare TC-32-1 | I1SB08 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| anion exchange column chromatography and gel filtration | Heterobasidion irregulare |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl beta-D-lactopyranoside + H2O | - |
Heterobasidion irregulare | 4-methylumbelliferone + lactose | - |
? | |
| 4-methylumbelliferyl beta-D-lactopyranoside + H2O | - |
Heterobasidion irregulare TC-32-1 | 4-methylumbelliferone + lactose | - |
? | |
| cellulose + H2O | - |
Heterobasidion irregulare | cellobiose + ? | - |
? | |
| cellulose + H2O | - |
Heterobasidion irregulare TC-32-1 | cellobiose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, SDS-PAGE | Heterobasidion irregulare |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cel7A | - |
Heterobasidion irregulare |
| cellobiohydrolase | - |
Heterobasidion irregulare |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Heterobasidion irregulare |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | about 45% activity at 30°C, about 19% activity at 50°C | Heterobasidion irregulare |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Heterobasidion irregulare |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | about 65% activity at pH 3.0, 71% activity at pH 5.0, 23% activityat pH 6.0 | Heterobasidion irregulare |
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