Literature summary for 3.2.1.176 extracted from

Dana, C.M.; Dotson-Fagerstrom, A.; Roche, C.M.; Kal, S.M.; Chokhawala, H.A.; Blanch, H.W.; Clark, D.S.
The importance of pyroglutamate in cellulase Cel7A (2014), Biotechnol. Bioeng., 111, 842-847.

Search for more literature for 3.2.1.176

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of C-terminally His6-tagged enzyme in Saccharomyces cerevisiae, and expression in Neurospora crassa using an Neurospora crassa codon bias and Neurospora crassa CBHI signal peptide and cloned in pCSR1:GPD vector, which directs gene integration to the csr-1 locus. Gene expression is promoted by the constitutive Myceliophthora thermophila gpdA promoter. The Cel7A expressed in Neurospora crassa has a higher melting temperature and higher specific activity than the Cel7A expressed in Saccharomyces cerevisiae. The underlying cause of this disparity is the lack of N-terminal glutamine cyclization in the Cel7A expressed in Saccharomyces cerevisiae. Treating the enzyme in vitro with glutaminyl cyclase improves the properties of Cel7A expressed in Saccharomyces cerevisiae to match those of Cel7A expressed in Neurospora crassa	Rasamsonia emersonii

Cloned (Comment)

Organism

recombinant expression of C-terminally His6-tagged enzyme in Saccharomyces cerevisiae, and expression in Neurospora crassa using an Neurospora crassa codon bias and Neurospora crassa CBHI signal peptide and cloned in pCSR1:GPD vector, which directs gene integration to the csr-1 locus. Gene expression is promoted by the constitutive Myceliophthora thermophila gpdA promoter. The Cel7A expressed in Neurospora crassa has a higher melting temperature and higher specific activity than the Cel7A expressed in Saccharomyces cerevisiae. The underlying cause of this disparity is the lack of N-terminal glutamine cyclization in the Cel7A expressed in Saccharomyces cerevisiae. Treating the enzyme in vitro with glutaminyl cyclase improves the properties of Cel7A expressed in Saccharomyces cerevisiae to match those of Cel7A expressed in Neurospora crassa

Rasamsonia emersonii

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	Cel7A tends to be strongly inhibited by its reaction products, is only moderately stable, and has a narrow pH range for activity	Rasamsonia emersonii

Organism

Organism	UniProt	Comment	Textmining
Rasamsonia emersonii	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
additional information	N-terminal glutamine cyclization in the Cel7A improves the properties of the enzyme concering thermal stability and enzyme activity	Rasamsonia emersonii

Purification (Commentary)

Purification (Comment)	Organism
recombinant C-terminally His6-tagged enzyme from Saccharomyces cerevisiae by anion exchange chromaatography and dialysis, recombinant enzyme from Neurospora crassa by ammonium sulfate fractionation, gel filtration, anion exchange chromatography and dialysis	Rasamsonia emersonii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	melting temperatures, recombinant expression in Saccharomyces cerevisiae and in Neurospora crassa. The Cel7A expressed in Neurospora crassa has a by 10°C higher melting temperature and higher specific activity than the Cel7A expressed in Saccharomyces cerevisiae	Rasamsonia emersonii

Synonyms

Synonyms	Comment	Organism
Cel7A	-	Rasamsonia emersonii

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
64.2	74.7	melting tempeartures, recombinant expression in Saccharomyces cerevisiae and in Neurospora crassa. The Cel7A expressed in Neurospora crassa has a by 10°C higher melting temperature and higher specific activity than the Cel7A expressed in Saccharomyces cerevisiae	Rasamsonia emersonii