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Literature summary for 3.2.1.176 extracted from

  • Heinzelman, P.; Komor, R.; Kanaan, A.; Romero, P.; Yu, X.; Mohler, S.; Snow, C.; Arnold, F.
    Efficient screening of fungal cellobiohydrolase class I enzymes for thermostabilizing sequence blocks by SCHEMA structure-guided recombination (2010), Protein Eng. Des. Sel., 23, 871-880.
    View publication on PubMed
Search for more literature for 3.2.1.176

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain YDR483W Trichoderma reesei
expressed in Saccharomyces cerevisiae strain YDR483W Thermoascus aurantiacus
expressed in Saccharomyces cerevisiae strain YDR483W Thermochaetoides thermophila
expressed in Saccharomyces cerevisiae strain YDR483W Rasamsonia emersonii
expressed in Saccharomyces cerevisiae strain YDR483W Acremonium thermophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellulose + H2O Trichoderma reesei
-
 ?
-
 ?
cellulose + H2O Thermoascus aurantiacus
-
 ?
-
 ?
cellulose + H2O Thermochaetoides thermophila
-
 ?
-
 ?
cellulose + H2O Rasamsonia emersonii
-
 ?
-
 ?
cellulose + H2O Acremonium thermophilum
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Acremonium thermophilum
-
-
-
Rasamsonia emersonii
-
-
-
Thermoascus aurantiacus
-
-
-
Thermochaetoides thermophila
-
-
-
Trichoderma reesei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ affinity column chromatography Trichoderma reesei
Ni2+ affinity column chromatography Thermoascus aurantiacus
Ni2+ affinity column chromatography Thermochaetoides thermophila
Ni2+ affinity column chromatography Rasamsonia emersonii
Ni2+ affinity column chromatography Acremonium thermophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl lactoside + H2O
-
 Trichoderma reesei 4-methylumbelliferone + lactose
-
 ?
4-methylumbelliferyl lactoside + H2O
-
 Thermoascus aurantiacus 4-methylumbelliferone + lactose
-
 ?
4-methylumbelliferyl lactoside + H2O
-
 Thermochaetoides thermophila 4-methylumbelliferone + lactose
-
 ?
4-methylumbelliferyl lactoside + H2O
-
 Rasamsonia emersonii 4-methylumbelliferone + lactose
-
 ?
cellulose + H2O
-
 Trichoderma reesei ?
-
 ?
cellulose + H2O
-
 Thermoascus aurantiacus ?
-
 ?
cellulose + H2O
-
 Thermochaetoides thermophila ?
-
 ?
cellulose + H2O
-
 Rasamsonia emersonii ?
-
 ?
cellulose + H2O
-
 Acremonium thermophilum ?
-
 ?

Synonyms

Synonyms Comment Organism
CBH I
-
 Trichoderma reesei
CBH I
-
 Thermoascus aurantiacus
CBH I
-
 Thermochaetoides thermophila
CBH I
-
 Rasamsonia emersonii
CBH I
-
 Acremonium thermophilum
cellobiohydrolase class I
-
 Trichoderma reesei
cellobiohydrolase class I
-
 Thermoascus aurantiacus
cellobiohydrolase class I
-
 Thermochaetoides thermophila
cellobiohydrolase class I
-
 Rasamsonia emersonii
cellobiohydrolase class I
-
 Acremonium thermophilum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 the enzyme is not active at temperatures higher than 65°C Trichoderma reesei
65
-
 the enzyme is not active at temperatures higher than 65°C Thermoascus aurantiacus
65
-
 the enzyme is not active at temperatures higher than 65°C Thermochaetoides thermophila
65
-
 the enzyme is not active at temperatures higher than 65°C Rasamsonia emersonii
65
-
 the enzyme is not active at temperatures higher than 65°C Acremonium thermophilum