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Literature summary for 3.2.1.176 extracted from

  • Voutilainen, S.P.; Boer, H.; Linder, M.B.; Puranen, T.; Rouvinen, J.; Vehmaanperae, J.; Koivula, A.
    Heterologous expression of Melanocarpus albomyces cellobiohydrolase Cel7B, and random mutagenesis to improve its thermostability (2007), Enzyme Microb. Technol., 41, 234-243.
No PubMed abstract available
Search for more literature for 3.2.1.176

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain NY179 Melanocarpus albomyces

Protein Variants

Protein Variants Comment Organism
A163T improved thermostability Melanocarpus albomyces
A30T mutant shows improved unfolding temperature by 1.5°C compared to the wild type enzyme (64.5°C) Melanocarpus albomyces
A3D improved thermostability Melanocarpus albomyces
D411G improved thermostability Melanocarpus albomyces
G184D mutant has higher specific activity and shows wild type unfolding temperature (64.5°C) Melanocarpus albomyces
G346D improved thermostability Melanocarpus albomyces
G386V improved thermostability Melanocarpus albomyces
G75D improved thermostability Melanocarpus albomyces
P114T improved thermostability Melanocarpus albomyces
R166Q improved thermostability Melanocarpus albomyces
S290T mutant has slightly lower specific activity and shows improved unfolding temperature by 3.5°C compared to the wild type enzyme (64.5°C) Melanocarpus albomyces
T56S improved thermostability Melanocarpus albomyces
Y100N improved thermostability Melanocarpus albomyces

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47500
-
 SDS-PAGE Melanocarpus albomyces

Organism

Organism UniProt Comment Textmining
Melanocarpus albomyces
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Sepharose FF column chromatography Melanocarpus albomyces

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-lactoside + H2O
-
 Melanocarpus albomyces 4-methylumbelliferone + lactose
-
 ?
avicel + H2O
-
 Melanocarpus albomyces cellobiose + ?
-
 ?

Synonyms

Synonyms Comment Organism
Cel7B
-
 Melanocarpus albomyces
cellobiohydrolase
-
 Melanocarpus albomyces

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Melanocarpus albomyces

pI Value

Organism Comment pI Value Maximum pI Value
Melanocarpus albomyces pI around 4, isoelectric focusing
-
 4