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Literature summary for 3.2.1.176 extracted from
- Biochemical and molecular characterization of a cellobiohydrolase from Trametes versicolor (2007), Appl. Microbiol. Biotechnol., 75, 337-346.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Aspergillus niger | Trametes versicolor |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.58 | - |
p-nitrophenyl beta-D-cellobioside | - |
Trametes versicolor |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
SDS-PAGE | Trametes versicolor |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trametes versicolor | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Superdex 75 gel filtration and Mono-Q column chromatography | Trametes versicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no detectable hydrolysis is observed with carboxymethylcellulose, laminarin and p-nitrophenyl beta-D-glucopyranoside | Trametes versicolor | ? | - |
? | |
| p-nitrophenyl beta-D-cellobioside + H2O | - |
Trametes versicolor | p-nitrophenol + cellobiose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CBH | - |
Trametes versicolor |
| Cel7A | - |
Trametes versicolor |
| cellobiohydrolase | - |
Trametes versicolor |
| exo-1,4-beta-glucanase | - |
Trametes versicolor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Trametes versicolor |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | unstable at temperatures lower than 50°C, retains about 80% of its activity after a 30 min incubation at 50°C and is completely inactivated after a 30 min pre-incubation at or above 60°C | Trametes versicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Trametes versicolor |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | - |
Trametes versicolor |
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Release 2023.1 (January 2023)
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