Crystallization (Comment) | Organism |
---|---|
deglycosylated Cel7D complexed with cellobiose, or with inhibitors cellobioimidazole, lactose, or methyl (4S)-beta-cellobiosyl-4-thio-beta-cellobioside, hanging drop vapour diffusion method, 18 mg/ml protein in 10 mM Tris-HCl, pH 7.0, 5 mM CaCl2, 15-22.5% PEG 5000, and 12% glycerol, soaking of crystals in ligand solution containing 10 mM ligand, X-ray diffraction structure determination and analysis at -173°C and 1.7-1.8 A resolution | Phanerodontia chrysosporium |
Protein Variants | Comment | Organism |
---|---|---|
E217Q | inactive mutant | Phanerodontia chrysosporium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Phanerodontia chrysosporium | |
cellobioimidazole | i.e. (5R,6R,7S,8S)-6-(beta-D-glucopyranosyloxy)-5,6,7,8-tetrahydro-5-[(hydroxy)methyl]imidazol[1,2a] pyridine-7,8-diol, the disaccharide binds in the glycosyl-binding subsites +1 and +2 close to the exit of the cellulose-binding tunnel/cleft of Cel7D, binding structure analysis | Phanerodontia chrysosporium | |
lactose | the disaccharide binds in the glycosyl-binding subsites +1 and +2 close to the exit of the cellulose-binding tunnel/cleft of Cel7D, binding structure analysis | Phanerodontia chrysosporium | |
methyl (4S)-beta-cellobiosyl-4-thio-beta-cellobioside | a thio-linked substrate analogue, the disaccharide binds in the glycosyl-binding subsites +1 and +2 close to the exit of the cellulose-binding tunnel/cleft of Cel7D, binding structure analysis | Phanerodontia chrysosporium | |
Tris | - |
Phanerodontia chrysosporium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Phanerodontia chrysosporium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion of Cel7D to the medium | Phanerodontia chrysosporium | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellulose + H2O | Phanerodontia chrysosporium | - |
cellobiose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | Q7LIJ0 | White Rot fungus, Cel7D is the major cellulase | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | Cel7D | Phanerodontia chrysosporium |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 cellohexaose + 2 H2O = 2 cellotriose + cellotetraose + cellobiose | substrate binding structure analysis of Cel7D | Phanerodontia chrysosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellulose + H2O | - |
Phanerodontia chrysosporium | cellobiose + ? | - |
? | |
additional information | substrate binding structure analysis of Cel7D | Phanerodontia chrysosporium | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cel7D | - |
Phanerodontia chrysosporium |
More | the enzyme Cel7D belongs to the glycoside hydrolase family 7 | Phanerodontia chrysosporium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Phanerodontia chrysosporium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Phanerodontia chrysosporium |