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Literature summary for 3.2.1.176 extracted from

  • Sanchez, M.M.; Pastor, F.I.; Diaz, P.
    Exo-mode of action of cellobiohydrolase Cel48C from Paenibacillus sp. BP-23. A unique type of cellulase among Bacillales (2003), Eur. J. Biochem., 270, 2913-2919.
    View publication on PubMed
Search for more literature for 3.2.1.176

Cloned(Commentary)

Cloned (Comment) Organism
-
 Paenibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
118000
-
 calculated from amino acid sequence Paenibacillus sp.
122000
-
 SDS-PAGE Paenibacillus sp.

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. Q8KKF7
-
-
Paenibacillus sp. BP-23 Q8KKF7
-
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Paenibacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00188
-
 bacterial microcrystalline cellulose as substrate Paenibacillus sp.
0.00488
-
 acid swollen cellulose as substrate Paenibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl cellopentaoside + H2O
-
 Paenibacillus sp. 4-nitrophenyl-cellobioside + cellotriose
-
 ?
4-nitrophenyl cellopentaoside + H2O
-
 Paenibacillus sp. BP-23 4-nitrophenyl-cellobioside + cellotriose
-
 ?
4-nitrophenyl cellotetraoside + H2O
-
 Paenibacillus sp. 4-nitrophenyl cellobioside + cellobiose
-
 ?
4-nitrophenyl cellotetraoside + H2O
-
 Paenibacillus sp. BP-23 4-nitrophenyl cellobioside + cellobiose
-
 ?
cellodextrin + H2O
-
 Paenibacillus sp. ?
-
 ?
cellodextrin + H2O
-
 Paenibacillus sp. BP-23 ?
-
 ?
cellulose + H2O acid-swollen cellulose, bacterial microcrystlline cellulose Paenibacillus sp. cellobiose + D-glucose + cellotriose
-
 ?
cellulose + H2O acid-swollen cellulose, bacterial microcrystlline cellulose Paenibacillus sp. BP-23 cellobiose + D-glucose + cellotriose
-
 ?
additional information no activity on carboxymethyl cellulose, cellobiose, cellotriose or 4-methylumbelliferyl alpha-D-glucoside Paenibacillus sp. ?
-
 ?
additional information no activity on carboxymethyl cellulose, cellobiose, cellotriose or 4-methylumbelliferyl alpha-D-glucoside Paenibacillus sp. BP-23 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Paenibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Paenibacillus sp.

pI Value

Organism Comment pI Value Maximum pI Value
Paenibacillus sp. calculated from amino acid sequence
-
 4.9