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Literature summary for 3.2.1.176 extracted from
- Cloning, expression and characterization of a family 48 exocellulase, Cel48A, from Thermobifida fusca (2000), Eur. J. Biochem., 267, 4988-4997.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned into Escherichia coli and Streptomyces lividans | Thermobifida fusca |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cellobiose | - |
Thermobifida fusca |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermobifida fusca | Q9XCD4 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | no glycoprotein | Thermobifida fusca |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| the enzyme and catalytic domain alone are each expressed in and purified from Streptomyces lividans | Thermobifida fusca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellopentaose + H2O | - |
Thermobifida fusca | cellotriose + cellobiose | - |
? | |
| cellotetraose + H2O | - |
Thermobifida fusca | 2 cellobiose | - |
? | |
| cellulose + H2O | - |
Thermobifida fusca | cellobiose + ? | - |
? | |
| additional information | Cel48A does not cleave cellotriose. No reaction at all with 4-methylumbelliferyl cellobioside and very low activity with 4-methylumbelliferyl cellotrioside | Thermobifida fusca | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cel48A | the enzyme is a family 48 exocellulase | Thermobifida fusca |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
17 h, 67% loss of activity | Thermobifida fusca |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | hydrolysis of swollen cellulose | Thermobifida fusca |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | pH 5: about 45% of maximal activity, pH 9.0: about 45% of maximal activity | Thermobifida fusca |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thermobifida fusca | isoelectric focusing | - |
4 |
| Thermobifida fusca | calculated from sequence | - |
4.2 |
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