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Literature summary for 3.2.1.176 extracted from
- Product inhibition of the recombinant CelS, and exoglucanase component of the Clostridium thermocellum cellulosome (1995), Appl. Microbiol. Biotechnol., 44, 399-404.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned in Escherichia coli | Acetivibrio thermocellus |
| expression in Escherichia coli | Acetivibrio thermocellus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arbutin | 20 mg/ml, 30% inhibition | Acetivibrio thermocellus |  |
| cellobiose | inhibits recombinant CelS activity on cellopentaose. Complete inhibition at 10 mg/ml cellobiose. Cellobiose appeared to inhibit the CelS activity through a competitive mechanism. The inhibition is relieved when beta-glucosidase is added, presumably because of the conversion of cellobiose into glucose | Acetivibrio thermocellus | |
| lactose | inhibits recombinant CelS activity on cellopentaose, 81% inhibition at 20 mg/ml lactose | Acetivibrio thermocellus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
cellopentaose | pH 5.7, temperature not specified in the publication | Acetivibrio thermocellus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetivibrio thermocellus | - |
- |
- |
| Acetivibrio thermocellus | A3DH67 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acetivibrio thermocellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| avicel + H2O | - |
Acetivibrio thermocellus | cellobiose | - |
? | |
| cellopentaose + H2O | - |
Acetivibrio thermocellus | cellobiose + cellotriose | - |
? | |
| cellulose + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
| cellulose + H2O | - |
Acetivibrio thermocellus | cellobiose + D-glucose + cellotriose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| celS | - |
Acetivibrio thermocellus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Acetivibrio thermocellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | - |
assay at | Acetivibrio thermocellus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | CelS is the exoglucanase component of the Clostridium thermocellum cellulosome multicomponent cellulase complex | Acetivibrio thermocellus |
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