Literature summary for 3.2.1.175 extracted from

Xu, Z.Y.; Lee, K.H.; Dong, T.; Jeong, J.C.; Jin, J.B.; Kanno, Y.; Kim, D.H.; Kim, S.Y.; Seo, M.; Bressan, R.A.; Yun, D.J.; Hwang, I.
A vacuolar beta-glucosidase homolog that possesses glucose-conjugated abscisic acid hydrolyzing activity plays an important role in osmotic stress responses in Arabidopsis (2012), Plant Cell, 24, 2184-2199.

Search for more literature for 3.2.1.175

Cloned(Commentary)

Cloned (Comment)	Organism
transient expression of HA-tagged wild-type enzyme and mutant E426Q in protoplasts, expression of GFP-tagged enzyme in vacuoles	Arabidopsis thaliana
transient expression of HA-tagged wild-type enzyme BG1 in protoplasts	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
E426Q	site-directed mutagenesis the enzyme is not induced by Glc-conjugated abscisic acid and shows reduced activity compared to the wild-type enzyme	Arabidopsis thaliana
additional information	overexpression of BG2 rescues the bg1 mutant phenotype, as observed for the overexpression of NCED3 in bg1 mutants. NaCl stress-sensitive root growth of bg2-1 and bg2-2 mutants	Arabidopsis thaliana
additional information	overexpression of NCED3 in bg1 mutants	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
vacuole	BG2 localizes to the central vacuole. The N-terminal region of enzyme BG2 is responsible for the vacuolar targeting. The majority of BG2 localizes to the vacuole	Arabidopsis thaliana	5773	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
98000	-	x * 98000, GFP-tagged enzyme, SDS-PAGE	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-
Arabidopsis thaliana	Q9SE50	-	-
Arabidopsis thaliana Col-0	-	-	-
Arabidopsis thaliana Col-0	Q9SE50	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant GFP-tagged enzyme from vacuoles by ultracentrifugation	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Glc-conjugated abscisic acid + H2O	in vitro activity of isozyme BG2	Arabidopsis thaliana	abscisic acid + D-glucose	-	?
Glc-conjugated abscisic acid + H2O	in vitro activity of isozyme BG2	Arabidopsis thaliana Col-0	abscisic acid + D-glucose	-	?

Subunits

Subunits	Comment	Organism
?	x * 98000, GFP-tagged enzyme, SDS-PAGE	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
beta-glucosidase homolog 1	-	Arabidopsis thaliana
beta-glucosidase homolog 2	-	Arabidopsis thaliana
BG1	-	Arabidopsis thaliana
BG2	-	Arabidopsis thaliana

Expression

Organism	Comment	Expression
Arabidopsis thaliana	the recombinant enzyme is induced by Glc-conjugated abscisic acid	up

General Information

General Information	Comment	Organism
malfunction	multiple Arabidopsis thaliana bg2 alleles with a T-DNA insertion in BG2 are more sensitive to dehydration and NaCl stress compared to the wild-type enzyme, whereas BG2 overexpression results in enhanced resistance to dehydration and NaCl stress	Arabidopsis thaliana
physiological function	in addition to the de novo biosynthesis, abscisic acid is produced in multiple organelles by organelle-specific beta-glucosidases in response to abiotic stresses. BG2 increases abscisic acid levels in protoplasts upon application of exogenous Glc-conjugated abscisic acid	Arabidopsis thaliana
physiological function	in Arabidopsis thaliana, abscisic acid levels are increased both through de novo biosynthesis and via beta-glucosidase homolog 1-mediated hydrolysis of Glc-conjugated abscisic acid. In addition to the de novo biosynthesis, abscisic acid is produced in multiple organelles by organelle-specific beta-glucosidases in response to abiotic stresses	Arabidopsis thaliana

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Release 2023.1 (January 2023)