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Literature summary for 3.2.1.175 extracted from

  • Lee, K.H.; Piao, H.L., Kim, H.Y.; Choi, S.M.; Jiang, F.; Hartung, W.; Hwang, I.; Kwak, J.M.; Lee, I.J.; Hwang, I.
    Activation of glucosidase via stress-induced polymerization rapidly increases active pools of abscisic acid (2006), Cell, 126, 1109-1120.
    View publication on PubMed
Search for more literature for 3.2.1.175

Protein Variants

Protein Variants Comment Organism
DELTAC105 a deletion mutant that lacks 105 residues at the C terminus. NO activity Arabidopsis thaliana
E207Q the amount of abscisate released by the mutant enzyme is reduced to 25% of that of the wild-type value, indicating that glutamic acid at position 207 is important for hydrolytic activity Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum abscisate generated by AtBG1 in the endoplasmic reticulum is secreted from cells into the apoplastic space of the leaf where it may play a role in initiation of ABA signaling Arabidopsis thaliana 5783
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 10 * 60000, main peak of AtBG1 in dehydration-stressed plants. Dehydration rapidly induces polymerization of AtBG1, resulting in a 4fold increase in enzymatic activity. Diurnal increases in abscisate levels are attributable to polymerization-mediated AtBG1 activation Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-glucopyranosyl abscisate + H2O Arabidopsis thaliana abscisic acid is a phytohormone critical for plant growth. Dehydration rapidly induces polymerization of the beta-D-glucopyranosyl abscisate hydrolyzing enzyme AtBG1, resulting in a 4fold increase in enzymatic activity. Diurnal increases in abscisate levels are attributable to polymerization-mediated AtBG1 activation. Activation of inactive abscisate pools by polymerized AtBG1 is a mechanism by which plants rapidly adjust abscisate levels and respond to changing environmental cues. Assembly of AtBG1 into higher molecular weight forms upon dehydration stress occurs at a mild condition such as exposure to 30% relative humidity for 30 min, indicating that the response is very sensitive to dehydration stress beta-D-glucopyranose + abscisate
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SE50 ecotype Columbia
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Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Arabidopsis thaliana
additional information dehydration rapidly induces polymerization of the beta-D-glucopyranosyl abscisate hydrolyzing enzyme AtBG1, resulting in a 4fold increase in enzymatic activity. Assembly of AtBG1 into higher molecular weight forms upon dehydration stress occurs at a mild condition such as exposure to 30% relative humidity for 30 min, indicating that the response is very sensitive to dehydration stress Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf hydathodes of rosette and cauline leaves Arabidopsis thaliana
-
seed
-
 Arabidopsis thaliana
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucopyranosyl abscisate + H2O abscisic acid is a phytohormone critical for plant growth. Dehydration rapidly induces polymerization of the beta-D-glucopyranosyl abscisate hydrolyzing enzyme AtBG1, resulting in a 4fold increase in enzymatic activity. Diurnal increases in abscisate levels are attributable to polymerization-mediated AtBG1 activation. Activation of inactive abscisate pools by polymerized AtBG1 is a mechanism by which plants rapidly adjust abscisate levels and respond to changing environmental cues. Assembly of AtBG1 into higher molecular weight forms upon dehydration stress occurs at a mild condition such as exposure to 30% relative humidity for 30 min, indicating that the response is very sensitive to dehydration stress Arabidopsis thaliana beta-D-glucopyranose + abscisate
-
 ?
beta-D-glucopyranosyl abscisate + H2O the enzyme is specific for beta-D-glucopyranosyl abscisate Arabidopsis thaliana beta-D-glucopyranose + abscisate
-
 ?
additional information the enzyme does not hydrolyze beta-D-glucopyranosyl zeatin Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
decamer 10 * 60000, main peak of AtBG1 in dehydration-stressed plants. Dehydration rapidly induces polymerization of AtBG1, resulting in a 4fold increase in enzymatic activity. Diurnal increases in abscisate levels are attributable to polymerization-mediated AtBG1 activation Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
ABA-GE hydrolyzing activity
-
 Arabidopsis thaliana
AtBG1
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction loss of AtBG1 causes defective stomatal movement, early germination, abiotic stress-sensitive phenotypes, and lower abscisate levels, whereas plants with ectopic AtBG1 accumulate higher abscisate levels and display enhanced tolerance to abiotic stress Arabidopsis thaliana
metabolism abscisic acid is a phytohormone critical for plant growth. Dehydration rapidly induces polymerization of the beta-D-glucopyranosyl abscisate hydrolyzing enzyme AtBG1, resulting in a 4fold increase in enzymatic activity. Diurnal increases in abscisate levels are attributable to polymerization-mediated AtBG1 activation. Activation of inactive abscisate pools by polymerized AtBG1 is a mechanism by which plants rapidly adjust abscisate levels and respond to changing environmental cues Arabidopsis thaliana