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Literature summary for 3.2.1.171 extracted from
- Rhamnogalacturonan I modifying enzymes an update (2016), New Biotechnol., 33, 41-54 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| phylogenetic analysis | Aspergillus aculeatus |
| phylogenetic analysis | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| rhamnogalacturonan I + H2O | Aspergillus aculeatus | RGI, degrading enzymes are active on the RGI backbone of pectin and are thus strictly specific for cleaving bonds in the repetitive [(1->2)-alpha-L-Rhap-(1->4)-alpha-D-GalpA-(1->2)] structure | ? | - |
? |  |
| rhamnogalacturonan I + H2O | Aspergillus niger | RGI, degrading enzymes are active on the RGI backbone of pectin and are thus strictly specific for cleaving bonds in the repetitive [(1->2)-alpha-L-Rhap-(1->4)-alpha-D-GalpA-(1->2)] structure | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus aculeatus | P87161 | - |
- |
| Aspergillus aculeatus | Q00001 | - |
- |
| Aspergillus niger | P87160 | - |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| Endohydrolysis of alpha-D-GalA-(1->2)-alpha-L-Rha glycosidic bond in the rhamnogalacturonan I backbone with initial inversion of anomeric configuration releasing oligosaccharides with beta-D-GalA at the reducing end | mode of action and site of action, overview. RGI endo-hydrolases, RGHs, attack the RGI backbone randomly in an endo-fashion and catalyse bond cleavage of alpha-(1->2) glycosidic bonds between D-GalpA and L-Rhap. The mechanism involves an inversion of the anomeric C1 configuration in galacturonic acid releasing oligosaccharides with beta-D-GalpA at the reducing end and L-Rhap at the non-reducing end as products | Aspergillus aculeatus | |
| Endohydrolysis of alpha-D-GalA-(1->2)-alpha-L-Rha glycosidic bond in the rhamnogalacturonan I backbone with initial inversion of anomeric configuration releasing oligosaccharides with beta-D-GalA at the reducing end | mode of action and site of action, overview. RGI endo-hydrolases, RGHs, attack the RGI backbone randomly in an endo-fashion and catalyse bond cleavage of alpha-(1->2) glycosidic bonds between D-GalpA and L-Rhap. The mechanism involves an inversion of the anomeric C1 configuration in galacturonic acid releasing oligosaccharides with beta-D-GalpA at the reducing end and L-Rhap at the non-reducing end as products | Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.4 | - |
substrate MHR-S, pH 4.5, 30°C | Aspergillus aculeatus |
| 0.9 | - |
substrate MHR-S, pH 4.5, 30°C | Aspergillus niger |
| 2.5 | - |
substrate MHR-S, pH 4.5, 30°C | Aspergillus aculeatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| MHR-S + H2O | i.e. saponified modified hairy regions of rhamnogalacturonan I, from apple pectin, residue remaining after enzymatic liquefaction of pectin | Aspergillus aculeatus | ? | - |
? | |
| MHR-S + H2O | i.e. saponified modified hairy regions of rhamnogalacturonan I, from apple pectin, residue remaining after enzymatic liquefaction of pectin | Aspergillus niger | ? | - |
? | |
| additional information | RGI endo-hydrolases, RGHs, attack the RGI backbone randomly in an endo-fashion and catalyse bond cleavage of alpha-(1->2) glycosidic bonds between D-GalpA and L-Rhap. The mechanism involves an inversion of the anomeric C1 configuration in galacturonic acid releasing oligosaccharides with beta-D-GalpA at the reducing end and L-Rhap at the non-reducing end as products | Aspergillus niger | ? | - |
? | |
| additional information | RGI endo-hydrolases, RGHs, attack the RGI backbone randomly in an endo-fashion and catalyse bond cleavage of alpha-(1->2) glycosidic bonds between D-GalpA and L-Rhap. The mechanism involves an inversion of the anomeric C1 configuration in galacturonic acid releasing oligosaccharides with beta-D-GalpA at the reducing end and L-Rhap at the non-reducing end as products | Aspergillus aculeatus | ? | - |
? | |
| additional information | RGI endo-hydrolases, RGHs, attack the RGI backbone randomly in an endo-fashion and catalyse bond cleavage of alpha-(1->2) glycosidic bonds between D-GalpA and L-Rhap. The mechanism involves an inversion of the anomeric C1 configuration in galacturonic acid releasing oligosaccharides with beta-D-GalpA at the reducing end and L-Rhap at the non-reducing end as products. Aspergillus aculeatus RhgA catalyses the hydrolytic cleavage of the alpha-D-GalpA-(1->2)-alpha-L-Rhap glycosidic linkages thus releasing (oligomeric) products with Rhap at the non-reducing end | Aspergillus aculeatus | ? | - |
? | |
| rhamnogalacturonan I + H2O | - |
Aspergillus aculeatus | ? | - |
? | |
| rhamnogalacturonan I + H2O | - |
Aspergillus niger | ? | - |
? | |
| rhamnogalacturonan I + H2O | RGI, degrading enzymes are active on the RGI backbone of pectin and are thus strictly specific for cleaving bonds in the repetitive [(1->2)-alpha-L-Rhap-(1->4)-alpha-D-GalpA-(1->2)] structure | Aspergillus aculeatus | ? | - |
? | |
| rhamnogalacturonan I + H2O | RGI, degrading enzymes are active on the RGI backbone of pectin and are thus strictly specific for cleaving bonds in the repetitive [(1->2)-alpha-L-Rhap-(1->4)-alpha-D-GalpA-(1->2)] structure | Aspergillus niger | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47000 | Aspergillus niger |
| ? | x * 46000 | Aspergillus aculeatus |
| ? | x * 58000 | Aspergillus aculeatus |
| More | structure analysis, overview | Aspergillus aculeatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RGH | - |
Aspergillus aculeatus |
| RGH | - |
Aspergillus niger |
| RGI endo-hydrolase | - |
Aspergillus aculeatus |
| RGI endo-hydrolase | - |
Aspergillus niger |
| RGI hydrolase | - |
Aspergillus aculeatus |
| RGI hydrolase | - |
Aspergillus niger |
| RhgA | - |
Aspergillus aculeatus |
| RhgA | - |
Aspergillus niger |
| RhgA_An | - |
Aspergillus niger |
| RhgB | - |
Aspergillus aculeatus |
| RhgB_An | - |
Aspergillus aculeatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | - |
Aspergillus aculeatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
- |
Aspergillus aculeatus |
| 3.6 | - |
- |
Aspergillus niger |
| 4.1 | - |
- |
Aspergillus aculeatus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus niger | - |
- |
4 |
| Aspergillus aculeatus | - |
- |
4.2 |
| Aspergillus aculeatus | - |
- |
4.6 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | phylogenetic and structural traits of RGI hydrolases, overview | Aspergillus aculeatus |
| evolution | phylogenetic and structural traits of RGI hydrolases, overview | Aspergillus niger |
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Release 2023.1 (January 2023)
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