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Literature summary for 3.2.1.171 extracted from
- Cloning and expression in Pichia pastoris of an Irpex lacteus rhamnogalacturonan hydrolase tolerant to acetylated rhamnogalacturonan (2012), Appl. Microbiol. Biotechnol., 94, 1543-1552.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Pichia pastoris X-33 | Irpex lacteus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme contains a secretion signal sequence of 22 amino acids | Irpex lacteus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44600 | - |
x * 44600, about, sequence calculation, x * 56000, recombinant enzyme, SDS-PAGE | Irpex lacteus |
| 56000 | - |
x * 44600, about, sequence calculation, x * 56000, recombinant enzyme, SDS-PAGE | Irpex lacteus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Irpex lacteus | B6E8Y7 | - |
- |
| Irpex lacteus KY2902 | B6E8Y7 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28.8 | - |
purified recombinant enzyme, substrate acetylated rhamnogalacturonan, pH 5.0, 40°C | Irpex lacteus |
| 75 | - |
purified recombinant enzyme, substrate non-acetylated rhamnogalacturonan, pH 5.0, 40°C | Irpex lacteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylated rhamnogalacturonan + H2O | - |
Irpex lacteus | ? | - |
? |  |
| acetylated rhamnogalacturonan + H2O | - |
Irpex lacteus KY2902 | ? | - |
? | |
| non-acetylated rhamnogalacturonan + H2O | - |
Irpex lacteus | ? | - |
? | |
| non-acetylated rhamnogalacturonan + H2O | - |
Irpex lacteus KY2902 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 44600, about, sequence calculation, x * 56000, recombinant enzyme, SDS-PAGE | Irpex lacteus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RG-hydrolase | - |
Irpex lacteus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | recombinant enzyme | Irpex lacteus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | activity range, overview | Irpex lacteus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
purified recombinant enzyme, stable up to | Irpex lacteus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 5 | recombinant enzyme | Irpex lacteus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | activity range, overview | Irpex lacteus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 6 | purified recombinant enzyme, 90 min 50% to 60% of the activity remaining | Irpex lacteus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Irpex lacteus | sequence calculation | - |
6.2 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycosyl hydrolase family 28, GH28 | Irpex lacteus |
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