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Literature summary for 3.2.1.17 extracted from
- Purification and characterization of recombinant human lysozyme from eggs of transgenic chickens (2015), PLoS ONE, 10, e0146032 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Gallus gallus eggs | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| peptidoglycan + H2O | Homo sapiens | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from eggs of transgenic chicken, Hiscreen SP column chromatography, Mono S column chromatography, and Superdex 75 gel filtration | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cell wall of Micrococcus lysodeikticus + H2O | - |
Homo sapiens | ? | - |
? | |
| peptidoglycan + H2O | - |
Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 14679, recombinant enzyme, MALDI-TOF mass spectrometry | Homo sapiens |
| ? | x * 14700, recombinant enzyme, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 100 | 15 min of treatment at 100°C disrupts almost all the antibacterial activity of the recombinant enzyme. Under 80°C, the recombinant enzyme can maintain about 60% of its activity for 15 min, and quickly loses the antibacterial activity after that. After incubation at 60°C for 45 min, the recombinant enzyme keeps 60% of its activity | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | - |
Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 11 | the recombinant enzyme retains more than 80% activity after 20 min at pH 2.0-11.0 | Homo sapiens |
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