Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Apostichopus japonicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Apostichopus japonicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA Sepharose column chromatography | Apostichopus japonicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
body wall | - |
Apostichopus japonicus | - |
coelomocyte | - |
Apostichopus japonicus | - |
intestine | lowest expression | Apostichopus japonicus | - |
respiratory system | - |
Apostichopus japonicus | - |
tentacle | highest expression | Apostichopus japonicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cell wall of Micrococcus lysodeikticus + H2O | - |
Apostichopus japonicus | ? | - |
? | |
additional information | the enzyme also has isopeptidase activity with 4-nitroanilide-L-gamma-glutamic acid | Apostichopus japonicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 14300, calculated from amino acid sequence | Apostichopus japonicus |
? | x * 32200, Thioredoxin-His-S-tagged enzyme, SDS-PAGE | Apostichopus japonicus |
Synonyms | Comment | Organism |
---|---|---|
iLys | - |
Apostichopus japonicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
optimum temperature for isopeptidase activity | Apostichopus japonicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
optimum pH for isopeptidase activity | Apostichopus japonicus |
Organism | Comment | Expression |
---|---|---|
Apostichopus japonicus | challenge with the pathogen Vibrio splendidus and lipopolysaccharide stimulation both significantly up-regulates the mRNA expression of the enzyme | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme serves as an important innate immunity factor and plays a key defense role during host-pathogen interactions in sea cucumbers | Apostichopus japonicus |