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Literature summary for 3.2.1.17 extracted from

  • Li, C.; Zhao, Y.; Liu, T.; Huang, J.; Zhang, Q.; Liu, B.; Xiao, S.; Wang, H.; Liu, B.; Wang, J.; Cong, L.
    The distribution and function characterization of the i type lysozyme from Apostichopus japonicus (2018), Fish Shellfish Immunol., 74, 419-425 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Apostichopus japonicus

Organism

Organism UniProt Comment Textmining
Apostichopus japonicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA Sepharose column chromatography Apostichopus japonicus

Source Tissue

Source Tissue Comment Organism Textmining
body wall
-
Apostichopus japonicus
-
coelomocyte
-
Apostichopus japonicus
-
intestine lowest expression Apostichopus japonicus
-
respiratory system
-
Apostichopus japonicus
-
tentacle highest expression Apostichopus japonicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cell wall of Micrococcus lysodeikticus + H2O
-
Apostichopus japonicus ?
-
?
additional information the enzyme also has isopeptidase activity with 4-nitroanilide-L-gamma-glutamic acid Apostichopus japonicus ?
-
?

Subunits

Subunits Comment Organism
? x * 14300, calculated from amino acid sequence Apostichopus japonicus
? x * 32200, Thioredoxin-His-S-tagged enzyme, SDS-PAGE Apostichopus japonicus

Synonyms

Synonyms Comment Organism
iLys
-
Apostichopus japonicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
optimum temperature for isopeptidase activity Apostichopus japonicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
optimum pH for isopeptidase activity Apostichopus japonicus

Expression

Organism Comment Expression
Apostichopus japonicus challenge with the pathogen Vibrio splendidus and lipopolysaccharide stimulation both significantly up-regulates the mRNA expression of the enzyme up

General Information

General Information Comment Organism
physiological function the enzyme serves as an important innate immunity factor and plays a key defense role during host-pathogen interactions in sea cucumbers Apostichopus japonicus