Cloned (Comment) | Organism |
---|---|
expression of enzyme mutants in Escherichia coli strain BL21(DE3) in inclusion bodies | Gallus gallus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | site-directed mutagenesis to modulate conformation and dynamic properties of the enzyme in non-native unfolded state, random-coil structure, modelling, and comprehensive analysis of non-native states of wild-type and mutant forms of the model protein lysozyme by nuclear magnetic resonance spectroscopy | Gallus gallus |
W108GA | site-directed mutagenesis | Gallus gallus |
W111GA | site-directed mutagenesis | Gallus gallus |
W123G | site-directed mutagenesis | Gallus gallus |
W28GA | site-directed mutagenesis | Gallus gallus |
W62GA | site-directed mutagenesis | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of recombinant mutants in unfolded non-native state, solubilization of the recombinant mutants from Escherichia coli strain BL21(DE3) inclusion bodies in 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, and 8 M urea, pH 7.5, centrifugation, and cation exchange chromatography with elution by 50 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, and 4 M urea, pH 7.5 | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Gallus gallus | - |
egg white | - |
Gallus gallus | - |