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Literature summary for 3.2.1.17 extracted from
- Overexpression and purification of recombinant lysozyme from Agrius convolvuli expressed as inclusion body in Escherichia coli (2012), Anim. Cells Sys., 16, 455-461.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of GST-tagged enzyme in inclusion body in Escherichia coli with induction of the fusion protein | Agrius convolvuli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrius convolvuli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-tagged enzyme from Escherichia coli inclusion bodies by solubilization and refolding, GST-tag cleavage through thrombin, and anion exchange chromatography and RPLC gel filtration | Agrius convolvuli |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| recombinant GST-tagged enzyme in inclusion bodies is resolubilized in solubilization buffer and dialyzed in refolding buffer | Agrius convolvuli |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | purified refolded recombinant enzyme exhibits antibacterial activity against Bacillus megaterium and Micrococcus luteus | Agrius convolvuli |
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