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Literature summary for 3.2.1.17 extracted from
- Purification and characterization of two bifunctional chitinases/lysozymes extracellulary produced by Pseudomonas aeruginosa K-187 in a shrimp and crab shell powder medium (1997), Appl. Environ. Microbiol., 63, 380-386.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Pseudomonas aeruginosa |  |
| dithiothreitol | - |
Pseudomonas aeruginosa | |
| glutathione | - |
Pseudomonas aeruginosa | |
| MgCl2 | - |
Pseudomonas aeruginosa | |
| Mn2+ | - |
Pseudomonas aeruginosa | |
| ZnCl2 | - |
Pseudomonas aeruginosa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | activates | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
FII, gel filtration | Pseudomonas aeruginosa |
| 32000 | - |
FII, 1 * 32000, SDS-PAGE | Pseudomonas aeruginosa |
| 60000 | - |
FI, gel filtration | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
K-187, two extracellular forms FI and FII | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.085 | - |
FII | Pseudomonas aeruginosa |
| 0.103 | - |
FI | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| colloidal chitin | - |
Pseudomonas aeruginosa | sugars | - |
? | |
| ethylene glycol chitin + H2O | - |
Pseudomonas aeruginosa | sugars | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 30000, SDS-PAGE | Pseudomonas aeruginosa |
| monomer | FII, 1 * 32000, SDS-PAGE | Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
FII | Pseudomonas aeruginosa |
| 50 | - |
FI | Pseudomonas aeruginosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
FI unaffected, 10 min | Pseudomonas aeruginosa |
| 60 | - |
FII, unaffected, 10 min | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
FII | Pseudomonas aeruginosa |
| 8 | - |
FI | Pseudomonas aeruginosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | FII | Pseudomonas aeruginosa |
| 6 | 9 | FI | Pseudomonas aeruginosa |
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