Literature summary for 3.2.1.169 extracted from

Li, B.; Li, H.; Hu, C.W.; Jiang, J.
Structural insights into the substrate binding adaptability and specificity of human O-GlcNAcase (2017), Nat. Commun., 8, 666 .

Search for more literature for 3.2.1.169

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta (DE3) cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
mutant enzyme D175N in complex with alpha-crystallin B, TAB1 protein, ELK1 protein or lamin B1, hanging drop vapor diffusion method, using 0.032 M ammonium citrate tribasic (pH 7.0), 0.02M MES monohydrate, 0.128 M potassium thiocyanate, 0.016M imidazole, 0.002 M zinc sulfate heptahydrate, 12.8% (w/v) polyethylene glycol 3350, 3.2% (w/v) polyethylene glycol monomethyl ether 2000, and 5% (w/v) polyethylene glycol monomethyl ether 550	Homo sapiens

Crystallization (Comment)

Organism

mutant enzyme D175N in complex with alpha-crystallin B, TAB1 protein, ELK1 protein or lamin B1, hanging drop vapor diffusion method, using 0.032 M ammonium citrate tribasic (pH 7.0), 0.02M MES monohydrate, 0.128 M potassium thiocyanate, 0.016M imidazole, 0.002 M zinc sulfate heptahydrate, 12.8% (w/v) polyethylene glycol 3350, 3.2% (w/v) polyethylene glycol monomethyl ether 2000, and 5% (w/v) polyethylene glycol monomethyl ether 550

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D175N	inactive	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O60502	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA resin column chromatography and Superdex S200 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
[alpha-crystallin B chain]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O	the sequence of alpha-crystallin B chain is FPTSTSLSPFYLR	Homo sapiens	[alpha-crystallin B chain]-L-serine + N-acetyl-D-glucosamine	-	?
[ELK1 protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O	the sequence of ELK1 protein is FWSTLSPI	Homo sapiens	[ELK1 protein]-L-threonine + N-acetyl-D-glucosamine	-	?
[lamin B1]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O	the sequence of lamin B1 protein is KLSPSPSSRVTVS	Homo sapiens	[lamin B1]-L-threonine + N-acetyl-D-glucosamine	-	?
[TAB1 protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O	the sequence of TAB1 protein is VPYSSAQS	Homo sapiens	[TAB1 protein]-L-serine + N-acetyl-D-glucosamine	-	?

Synonyms

Synonyms	Comment	Organism
O-GlcNAcase	-	Homo sapiens
O-linked beta-N-acetyl glucosaminase	-	Homo sapiens
OGA	-	Homo sapiens