Any feedback?
Literature summary for 3.2.1.169 extracted from
- Insights into activity and inhibition from the crystal structure of human O-GlcNAcase (2017), Nat. Chem. Biol., 13, 613-615 .
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| enzyme in apo and inhibited states, hanging drop vapor diffusion method | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| PUGNAc | i.e. O-(2-acetamido-2-deoxy-D-glucopyranosylidenamino) N-phenylcarbamate | Homo sapiens |  |
| thiamet G | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA57-400/544-705, pH and temperature not specified in the publication | Homo sapiens | |
| 0.07 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-400/554-705, pH and temperature not specified in the publication | Homo sapiens | |
| 0.17 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | full-length enzyme, pH and temperature not specified in the publication | Homo sapiens | |
| 0.81 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA1-662, pH and temperature not specified in the publication | Homo sapiens | |
| 2.1 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-400, pH and temperature not specified in the publication | Homo sapiens | |
| 4 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-341, pH and temperature not specified in the publication | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 103000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O | Homo sapiens | - |
[protein]-L-serine + N-acetyl-D-glucosamine | - |
? | |
| [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O | Homo sapiens | - |
[protein]-L-threonine + N-acetyl-D-glucosamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O60502 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-Sepharose column chromatography | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Homo sapiens | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
| [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O | - |
Homo sapiens | [protein]-L-serine + N-acetyl-D-glucosamine | - |
? | |
| [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O | - |
Homo sapiens | [protein]-L-threonine + N-acetyl-D-glucosamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | x-ray crystallography | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| O-GlcNAc hydrolase | - |
Homo sapiens |
| OGA | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-400, pH and temperature not specified in the publication | Homo sapiens | |
| 0.1 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA1-662, pH and temperature not specified in the publication | Homo sapiens | |
| 0.13 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-341, pH and temperature not specified in the publication | Homo sapiens | |
| 0.2 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-400/554-705, pH and temperature not specified in the publication | Homo sapiens | |
| 1.5 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA57-400/544-705, pH and temperature not specified in the publication | Homo sapiens | |
| 1.8 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | full-length enzyme, pH and temperature not specified in the publication | Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00000008 | - |
thiamet G | truncated enzyme OGA57-400/544-705, pH and temperature not specified in the publication | Homo sapiens | |
| 0.00000025 | - |
thiamet G | full-length enzyme, pH and temperature not specified in the publication | Homo sapiens | |
| 0.00000043 | - |
thiamet G | truncated enzyme OGA14-400/554-705, pH and temperature not specified in the publication | Homo sapiens | |
| 0.0000058 | - |
thiamet G | truncated enzyme OGA1-662, pH and temperature not specified in the publication | Homo sapiens | |
| 0.0000066 | - |
PUGNAc | truncated enzyme OGA57-400/544-705, pH and temperature not specified in the publication | Homo sapiens | |
| 0.0000123 | - |
PUGNAc | truncated enzyme OGA14-400/554-705, pH and temperature not specified in the publication | Homo sapiens | |
| 0.00004 | - |
PUGNAc | full-length enzyme, pH and temperature not specified in the publication | Homo sapiens | |
| 0.00059 | - |
PUGNAc | truncated enzyme OGA1-662, pH and temperature not specified in the publication | Homo sapiens | |
| 0.0031 | - |
thiamet G | truncated enzyme OGA14-400, pH and temperature not specified in the publication | Homo sapiens | |
| 0.009 | - |
thiamet G | truncated enzyme OGA14-341, pH and temperature not specified in the publication | Homo sapiens | |
| 0.011 | - |
PUGNAc | truncated enzyme OGA14-400, pH and temperature not specified in the publication | Homo sapiens | |
| 0.06 | - |
PUGNAc | truncated enzyme OGA14-341, pH and temperature not specified in the publication | Homo sapiens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-341, pH and temperature not specified in the publication | Homo sapiens | |
| 0.04 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-400, pH and temperature not specified in the publication | Homo sapiens | |
| 0.12 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA1-662, pH and temperature not specified in the publication | Homo sapiens | |
| 2.9 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-400/554-705, pH and temperature not specified in the publication | Homo sapiens | |
| 11 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | full-length enzyme, pH and temperature not specified in the publication | Homo sapiens | |
| 37 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA57-400/544-705, pH and temperature not specified in the publication | Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
