Crystallization (Comment) | Organism |
---|---|
enzyme in apo and inhibited states, hanging drop vapor diffusion method | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
PUGNAc | i.e. O-(2-acetamido-2-deoxy-D-glucopyranosylidenamino) N-phenylcarbamate | Homo sapiens | |
thiamet G | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA57-400/544-705, pH and temperature not specified in the publication | Homo sapiens | |
0.07 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-400/554-705, pH and temperature not specified in the publication | Homo sapiens | |
0.17 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | full-length enzyme, pH and temperature not specified in the publication | Homo sapiens | |
0.81 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA1-662, pH and temperature not specified in the publication | Homo sapiens | |
2.1 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-400, pH and temperature not specified in the publication | Homo sapiens | |
4 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-341, pH and temperature not specified in the publication | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
103000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O | Homo sapiens | - |
[protein]-L-serine + N-acetyl-D-glucosamine | - |
? | |
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O | Homo sapiens | - |
[protein]-L-threonine + N-acetyl-D-glucosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O60502 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Homo sapiens | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O | - |
Homo sapiens | [protein]-L-serine + N-acetyl-D-glucosamine | - |
? | |
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O | - |
Homo sapiens | [protein]-L-threonine + N-acetyl-D-glucosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | x-ray crystallography | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
O-GlcNAc hydrolase | - |
Homo sapiens |
OGA | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-400, pH and temperature not specified in the publication | Homo sapiens | |
0.1 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA1-662, pH and temperature not specified in the publication | Homo sapiens | |
0.13 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-341, pH and temperature not specified in the publication | Homo sapiens | |
0.2 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-400/554-705, pH and temperature not specified in the publication | Homo sapiens | |
1.5 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA57-400/544-705, pH and temperature not specified in the publication | Homo sapiens | |
1.8 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | full-length enzyme, pH and temperature not specified in the publication | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000008 | - |
thiamet G | truncated enzyme OGA57-400/544-705, pH and temperature not specified in the publication | Homo sapiens | |
0.00000025 | - |
thiamet G | full-length enzyme, pH and temperature not specified in the publication | Homo sapiens | |
0.00000043 | - |
thiamet G | truncated enzyme OGA14-400/554-705, pH and temperature not specified in the publication | Homo sapiens | |
0.0000058 | - |
thiamet G | truncated enzyme OGA1-662, pH and temperature not specified in the publication | Homo sapiens | |
0.0000066 | - |
PUGNAc | truncated enzyme OGA57-400/544-705, pH and temperature not specified in the publication | Homo sapiens | |
0.0000123 | - |
PUGNAc | truncated enzyme OGA14-400/554-705, pH and temperature not specified in the publication | Homo sapiens | |
0.00004 | - |
PUGNAc | full-length enzyme, pH and temperature not specified in the publication | Homo sapiens | |
0.00059 | - |
PUGNAc | truncated enzyme OGA1-662, pH and temperature not specified in the publication | Homo sapiens | |
0.0031 | - |
thiamet G | truncated enzyme OGA14-400, pH and temperature not specified in the publication | Homo sapiens | |
0.009 | - |
thiamet G | truncated enzyme OGA14-341, pH and temperature not specified in the publication | Homo sapiens | |
0.011 | - |
PUGNAc | truncated enzyme OGA14-400, pH and temperature not specified in the publication | Homo sapiens | |
0.06 | - |
PUGNAc | truncated enzyme OGA14-341, pH and temperature not specified in the publication | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-341, pH and temperature not specified in the publication | Homo sapiens | |
0.04 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-400, pH and temperature not specified in the publication | Homo sapiens | |
0.12 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA1-662, pH and temperature not specified in the publication | Homo sapiens | |
2.9 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA14-400/554-705, pH and temperature not specified in the publication | Homo sapiens | |
11 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | full-length enzyme, pH and temperature not specified in the publication | Homo sapiens | |
37 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | truncated enzyme OGA57-400/544-705, pH and temperature not specified in the publication | Homo sapiens |