Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | addition of Triton X-100 does not increase activity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(acetylamino)-2-deoxy-1-S-hexanoyl-1-thio-beta-D-glucopyranose | inhibits activity at 10 mM, 47.5% relative activity compared with activity without any addition of effector | Rattus norvegicus | |
2-acetamido-2-deoxy-D-gluconhydroxime-1,5-lactone | inhibits activity at 0.1 mM, 6.7% relative activity compared with activity without any addition of effector | Rattus norvegicus | |
2-acetamindo-1-amino-1,2-dideoxy-beta-D-glucopyranose | inhibits activity at 10 mM, 3.2% relative activity compared with activity without any addition of effector | Rattus norvegicus | |
2-acetamindo-1-azido-1,2-dideoxy-beta-D-glucopyranose | inhibits activity at 10 mM, 19.3% relative activity compared with activity without any addition of effector | Rattus norvegicus | |
2-acetamindo-1-bromo-1,2-dideoxy-beta-D-glucopyranose | inhibits activity at 10 mM, 34.8% relative activity compared with activity without any addition of effector | Rattus norvegicus | |
alpha-D-mannose | inhibits activity at 10 mM, 62.6% relative activity compared with activity without any addition of effector | Rattus norvegicus | |
castanospermine | inhibits activity at 10 mM, 32.7% relative activity compared with activity without any addition of effector | Rattus norvegicus | |
Cd2+ | inhibits activity at 1 mM, 7.7% relative activity compared with activity without any addition of effector | Rattus norvegicus | |
Cu2+ | inhibits activity at 1 mM, 1% relative activity compared with activity without any addition of effector | Rattus norvegicus | |
D-glucosamine | inhibits activity at 10 mM, 60.7% relative activity compared with activity without any addition of effector | Rattus norvegicus | |
deoxynojirimycin | slight inhibition | Rattus norvegicus | |
Hg2+ | inhibits activity at 1 mM, 6% relative activity compared with activity without any addition of effector | Rattus norvegicus | |
methyl alpha-D-mannose | inhibits activity at 10 mM, 11.3% relative activity compared with activity without any addition of effector. Other sugars and sugar analogs do not inhibit with exception of methyl-alpha-D-mannose | Rattus norvegicus | |
additional information | addition of 50 mM GalNAc does not inhibit the neutral hexosaminidase activity; GlcNAc analogs with no or small effect on the enzyme activity: N-acetylgalactosamine, 4-aminophenyl 1-thio-beta-D-galactopyranoside, (2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl)-L-asparagine, 2-(acetylamino)-2-deoxy-N-hexanoyl-beta-D-glucopyranosylamine, 2-(acetylamino)-2-deoxy-1-O-phosphono-beta-D-glucopyranose, bromoconduritol, iodoacetic acid, N-acetylneuraminic acid | Rattus norvegicus | |
N-acetyl-D-glucosamine | inhibits activity at 10 mM, 40% relative activity compared with activity without any addition of effector | Rattus norvegicus | |
N-ethylmaleimide | inhibits activity at 10 mM, 15.3% relative activity compared with activity without any addition of effector | Rattus norvegicus | |
N-methyl-deoxynojirimycin | inhibits activity at 10 mM, 73.5% relative activity compared with activity without any addition of effector | Rattus norvegicus | |
NaCl | high salt, 1.0 M NaCl, inhibits enzyme activity by 50% compared with activity without any addition of effector | Rattus norvegicus | |
O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate | inhibits activity at 0.1 mM, 4.1% relative activity compared with activity without any addition of effector | Rattus norvegicus | |
p-aminophenyl-S-GlcNAc | inhibits activity at 10 mM, 76.3% relative activity compared with activity without any addition of effector | Rattus norvegicus | |
Sn2+ | inhibits activity at 1 mM, 57.6% relative activity compared with activity without any addition of effector | Rattus norvegicus | |
Tris | high salt, 75 mM, inhibits enzyme activity by 50% compared with activity without any addition of effector | Rattus norvegicus | |
UDP-GlcNAc | inhibits activity at 10 mM, 78.1% relative activity compared with activity without any addition of effector | Rattus norvegicus | |
Zn2+ | inhibits activity at 1 mM, 7.3% relative activity compared with activity without any addition of effector | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | 5 | 4-nitrophenyl-beta-D-GlcNAc | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
nucleoplasm | enriched in nuclei | Rattus norvegicus | 5654 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | inhibits activity at 1 mM | Rattus norvegicus | |
Cu2+ | inhibits activity at 1 mM | Rattus norvegicus | |
Hg2+ | inhibits activity at 1 mM | Rattus norvegicus | |
additional information | EDTA, Mg2+, Fe3+, Ca2+, Mn2+, and Co2+ do not affect the activity | Rattus norvegicus | |
Zn2+ | inhibits activity at 1 mM | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
1 * 54000 (alpha-subunit) and 1 * 51000 (beta-subunit) | Rattus norvegicus |
54000 | - |
1 * 54000 (alpha-subunit) and 1 * 51000 (beta-subunit) | Rattus norvegicus |
106000 | - |
SDS-PAGE and sucrose density gradient | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | strong selectivity for O-GlcNAc-synthetic glycopeptides. Enzyme does not cleave 4-nitrophenylGalNAc and is highly selective for O-GlcNAc bearing glycopeptides. O-GlcNAcase is involved in the regulated removal of O-GlcNAc from O-GlcNAc-bearing glycoproteins in the nucleoplasmic and cytoplasmic compartments of cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
over 22000fold, gel filtration chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
spleen | enzyme is ubiquitously present in all rat tissues | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
last purification step: 13046.67 unit/mg. One unit is the amount of enzyme required to catalyze the release of 4-nitrophenyl from 1 mmol of 4-nitrophenylGlcNAc/min at 37°C | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminophenyl 2-(acetylamino)-2-deoxy-beta-D-glucopyranoside + H2O | 67% relative activity compared to 4-nitrophenyl-beta-D-GlcNac | Rattus norvegicus | ? | - |
? | |
4-methylumbelliferyl 2-(acetylamino)-2-deoxy-beta-D-glucopyranoside + H2O | 33% relative activity compared to 4-nitrophenyl-beta-D-GlcNac | Rattus norvegicus | ? | - |
? | |
4-nitrophenyl beta-D-GlcNAc + H2O | - |
Rattus norvegicus | 4-nitrophenol + N-acetyl-D-glucosamine | - |
? | |
GlcNAc beta(1-4)GlcNAc + H2O | 4% relative activity. Enzyme can cleave terminal Glc-NAc from disaccharides to a limited extent as long as GlcNAc is linked to the other saccharides beta-linkage at the C-1 position | Rattus norvegicus | N-acetyl-D-glucosamine + N-acetyl-D-glucosamine | - |
? | |
GlcNAc beta(1-6)Gal + H2O | 29% relative activity. Enzyme can cleave terminal Glc-NAc from disaccharides to a limited extent as long as GlcNAc is linked to the other saccharides beta-linkage at the C-1 position | Rattus norvegicus | N-acetyl-D-glucosamine + beta-D-galactose | - |
? | |
GlcNAcbeta(1-6)Man-beta-1-OMe + H2O | 33% relative activity. Enzyme can cleave terminal Glc-NAc from disaccharides to a limited extent as long as GlcNAc is linked to the other saccharides beta-linkage at the C-1 position | Rattus norvegicus | N-acetyl-D-glucosamine + methyl beta-D-mannoside | - |
? | |
additional information | strong selectivity for O-GlcNAc-synthetic glycopeptides. Enzyme does not cleave 4-nitrophenylGalNAc and is highly selective for O-GlcNAc bearing glycopeptides. O-GlcNAcase is involved in the regulated removal of O-GlcNAc from O-GlcNAc-bearing glycoproteins in the nucleoplasmic and cytoplasmic compartments of cells | Rattus norvegicus | ? | - |
? | |
additional information | shows no activity against other monosaccharides either in alpha or beta linkage to 4-nitrophenyl, such as, 4-nitrophenyl-alpha-D-GlcNAc, 4-nitrophenyl-alpha-D-glucose, 4-nitrophenyl-beta-D-glucose, 4-nitrophenyl-alpha-D-GalNAc, 4-nitrophenyl-beta-D-GalNAc, 4-nitrophenyl-alpha-D-galactose, 4-nitrophenyl-beta-D-galactose, 4-nitrophenyl-alpha-D-mannose, 4-nitrophenyl-alpha-L-fructose | Rattus norvegicus | ? | - |
? | |
additional information | substitution of GlcNAc at other positions prevents cleavage, for example, Gal(1-3)GlcNAc-p-nitrophenol, Gal(1-4)GlcNAc, Gal(1-3)GlcNAc, and UDP-GlcNAc, the enzyme specifically cleave GlcNAc at C-1 position in beta-linkage to other groups | Rattus norvegicus | ? | - |
? | |
P-(GlcNAc)STST + H2O | - |
Rattus norvegicus | PSTST + N-acetyl-D-glucosamine | - |
? | |
YSD-(GlcNAc)SPSTST + H2O | - |
Rattus norvegicus | YSDSPSTST + N-acetyl-D-glucosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 54000 (alpha-subunit) and 1 * 51000 (beta-subunit) | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
cytoplasmic O-GlcNAcase | - |
Rattus norvegicus |
neutral O-GlcNAcase | - |
Rattus norvegicus |
O-GlcNAc selective N-acetyl-beta-D-glucosaminidase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at and most active at | Rattus norvegicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 37 | most active at 37°C, and less active at room temperature, and very little activity at 4°C | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 37 | enzyme loses activity very fast (within 5 min) at 57°C, stable at 37°C and room temperature | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
at pH below 5.0, it begins to lose activity dramatically, at pH 6 or higher, it maintains high activity | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
enzyme is unstable below pH 4.5 | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000052 | - |
O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate | - |
Rattus norvegicus | |
0.00168 | - |
2-acetamido-2-deoxy-D-gluconhydroxime-1,5-lactone | - |
Rattus norvegicus | |
0.151 | - |
2-acetamindo-1-amino-1,2-dideoxy-beta-D-glucopyranose | - |
Rattus norvegicus | |
1.14 | - |
2-acetamindo-1-azido-1,2-dideoxy-beta-D-glucopyranose | - |
Rattus norvegicus | |
3.9 | - |
N-acetyl-D-glucosamine | - |
Rattus norvegicus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rattus norvegicus | - |
- |
5.7 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00015 | - |
- |
Rattus norvegicus | O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate | |
0.007 | - |
- |
Rattus norvegicus | 2-acetamido-2-deoxy-D-gluconhydroxime-1,5-lactone | |
0.16 | - |
- |
Rattus norvegicus | 2-acetamindo-1-amino-1,2-dideoxy-beta-D-glucopyranose | |
1.5 | - |
- |
Rattus norvegicus | 2-acetamindo-1-azido-1,2-dideoxy-beta-D-glucopyranose | |
7 | - |
- |
Rattus norvegicus | N-acetyl-D-glucosamine | |
30 | - |
- |
Rattus norvegicus | D-glucosamine |