Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | GlcN is found to provoke hyperglycemia. The insulin resistance index (HOMA-IR) increased. The beta-cell function index (HOMA-beta) diminished. Insulin release index (insulin/glucose) is less in the GlcN group than in the control. Muscle O-GlcNAcase activity diminished | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GalNAc | an inhibitor of other hexosaminidases | Rattus norvegicus | |
additional information | GlcN-induced whole-body insulin resistance is related to tissue O-GlcNAcase activity and mRNA expression. O-GlcNAcase activity is posttranslationally inhibited during GlcN-induced insulin resistance | Rattus norvegicus | |
O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenyl carbamate | PUGNAc | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.998 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | in the pancreas of rats treated with GlcN | Rattus norvegicus | |
1 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | compared to the control, pancreas of rats treated with saline | Rattus norvegicus | |
1.12 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | compared to the control, muscles of rats treated with saline | Rattus norvegicus | |
1.24 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | in the liver of rats treated with GlcN | Rattus norvegicus | |
1.29 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | compared to the control, liver of rats treated with saline | Rattus norvegicus | |
1.51 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | in the muscels of rats treated with GlcN | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | removes O-N-acetyl-glucosamine residues from O-GlcNAcylated proteins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Sprague-Dawley male rats. GlcN (30 micromol/kg/min) or physiological saline (control) is intravenously infused into the rats for 2 h | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
muscle | - |
Rattus norvegicus | - |
pancreas | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
in the rats treated with GlcN, muscle O-GlcNAcase activity diminished (0.28 vs. 0.36 nmol of p-nitrophenyl/mg protein/min) | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Rattus norvegicus | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
additional information | removes O-N-acetyl-glucosamine residues from O-GlcNAcylated proteins | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
O-GlcNAc-selective-N-acetyl-beta-D-glucosaminidase | - |
Rattus norvegicus |
O-GlcNAcase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |