Cloned (Comment) | Organism |
---|---|
soluble BtGH84 is expressed in the cytoplasm of Escherichia coli | Bacteroides thetaiotaomicron |
Crystallization (Comment) | Organism |
---|---|
X-ray crystal structure of BtGH84 in complex with the inhibitor NAG-thiazoline. Hanging drop vapor diffusion method | Bacteroides thetaiotaomicron |
Protein Variants | Comment | Organism |
---|---|---|
D242A | site-directed mutant, 2800fold lower catalytic efficiency than wild-type | Bacteroides thetaiotaomicron |
D242N | site-directed mutant | Bacteroides thetaiotaomicron |
D243A | site-directed mutant, 110fold lower catalytic efficiency than wild-type | Bacteroides thetaiotaomicron |
D243N | site-directed mutant | Bacteroides thetaiotaomicron |
N372A | site-directed mutant | Bacteroides thetaiotaomicron |
Y137F | site-directed mutant | Bacteroides thetaiotaomicron |
Y282F | site-directed mutant | Bacteroides thetaiotaomicron |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,2-dideoxy-2'-methyl-alpha-D-glucopyranoso-[2,1-d]-DELTA2'-thiazoline | NAG-thiazoline | Bacteroides thetaiotaomicron | |
O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate | PUGNAc | Bacteroides thetaiotaomicron | |
streptozotocin | STZ | Bacteroides thetaiotaomicron |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | D243N mutant | Bacteroides thetaiotaomicron | |
0.092 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | D243A mutant | Bacteroides thetaiotaomicron | |
0.28 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | wild-type | Bacteroides thetaiotaomicron | |
0.3 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | - |
Bacteroides thetaiotaomicron | |
0.41 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | Y282F mutant | Bacteroides thetaiotaomicron | |
1.18 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | Y137F mutant | Bacteroides thetaiotaomicron | |
1.4 | - |
4-nitrophenyl N-acetyl-beta-D-thioglucosaminide | - |
Bacteroides thetaiotaomicron | |
1.47 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | D242N mutant | Bacteroides thetaiotaomicron | |
4.4 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | D242A mutant | Bacteroides thetaiotaomicron | |
36 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | N372A mutant | Bacteroides thetaiotaomicron |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bacteroides thetaiotaomicron | enzyme cleaves 4-nitrophenyl N-acetyl-beta-D-glucosaminide from modified proteins and also cleaves 4-nitrophenyl N-acetyl-beta-D-thioglucosaminide | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides thetaiotaomicron | Q89ZI2 | - |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography using nickel-affinity chromatography followed by gel filtration | Bacteroides thetaiotaomicron |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O | - |
Bacteroides thetaiotaomicron | 4-methylumbelliferone + N-acetyl-beta-D-glucosamine | - |
? | |
4-methylumbelliferyl N-difluoroacetyl-beta-D-glucosaminide + H2O | - |
Bacteroides thetaiotaomicron | 4-methylumbelliferone + N-difluoroacetyl-beta-D-glucosamine | - |
? | |
4-methylumbelliferyl N-fluoroacetyl-beta-D-glucosaminide + H2O | - |
Bacteroides thetaiotaomicron | 4-methylumbelliferone + N-fluoroacetyl-beta-D-glucosamine | - |
? | |
4-methylumbelliferyl N-trifluoroacetyl-beta-D-glucosaminide + H2O | - |
Bacteroides thetaiotaomicron | 4-methylumbelliferone + N-trifluoroacetyl-beta-D-glucosamine | - |
? | |
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Bacteroides thetaiotaomicron | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
4-nitrophenyl N-acetyl-beta-D-thioglucosaminide + H2O | - |
Bacteroides thetaiotaomicron | 4-nitrophenol + N-acetyl-beta-D-thioglucosamine | - |
? | |
additional information | enzyme cleaves 4-nitrophenyl N-acetyl-beta-D-glucosaminide from modified proteins and also cleaves 4-nitrophenyl N-acetyl-beta-D-thioglucosaminide | Bacteroides thetaiotaomicron | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Bacteroides thetaiotaomicron |
Synonyms | Comment | Organism |
---|---|---|
BtGH84 | - |
Bacteroides thetaiotaomicron |
O-GlcNAcase | - |
Bacteroides thetaiotaomicron |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacteroides thetaiotaomicron |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
bell-shaped pH-activity profile | Bacteroides thetaiotaomicron |