Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.169 extracted from

  • Dennis, R.J.; Taylor, E.J.; Macauley, M.S.; Stubbs, K.A.; Turkenburg, J.P.; Hart, S.J.; Black, G.N.; Vocadlo, D.J.; Davies, G.J.
    Structure and mechanism of a bacterial beta -glucosaminidase having O-GlcNAcase activity (2006), Nat. Struct. Mol. Biol., 13, 365-371.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
soluble BtGH84 is expressed in the cytoplasm of Escherichia coli Bacteroides thetaiotaomicron

Crystallization (Commentary)

Crystallization (Comment) Organism
X-ray crystal structure of BtGH84 in complex with the inhibitor NAG-thiazoline. Hanging drop vapor diffusion method Bacteroides thetaiotaomicron

Protein Variants

Protein Variants Comment Organism
D242A site-directed mutant, 2800fold lower catalytic efficiency than wild-type Bacteroides thetaiotaomicron
D242N site-directed mutant Bacteroides thetaiotaomicron
D243A site-directed mutant, 110fold lower catalytic efficiency than wild-type Bacteroides thetaiotaomicron
D243N site-directed mutant Bacteroides thetaiotaomicron
N372A site-directed mutant Bacteroides thetaiotaomicron
Y137F site-directed mutant Bacteroides thetaiotaomicron
Y282F site-directed mutant Bacteroides thetaiotaomicron

Inhibitors

Inhibitors Comment Organism Structure
1,2-dideoxy-2'-methyl-alpha-D-glucopyranoso-[2,1-d]-DELTA2'-thiazoline NAG-thiazoline Bacteroides thetaiotaomicron
O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate PUGNAc Bacteroides thetaiotaomicron
streptozotocin STZ Bacteroides thetaiotaomicron

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide D243N mutant Bacteroides thetaiotaomicron
0.092
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide D243A mutant Bacteroides thetaiotaomicron
0.28
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide wild-type Bacteroides thetaiotaomicron
0.3
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide
-
Bacteroides thetaiotaomicron
0.41
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide Y282F mutant Bacteroides thetaiotaomicron
1.18
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide Y137F mutant Bacteroides thetaiotaomicron
1.4
-
4-nitrophenyl N-acetyl-beta-D-thioglucosaminide
-
Bacteroides thetaiotaomicron
1.47
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide D242N mutant Bacteroides thetaiotaomicron
4.4
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide D242A mutant Bacteroides thetaiotaomicron
36
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide N372A mutant Bacteroides thetaiotaomicron

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacteroides thetaiotaomicron enzyme cleaves 4-nitrophenyl N-acetyl-beta-D-glucosaminide from modified proteins and also cleaves 4-nitrophenyl N-acetyl-beta-D-thioglucosaminide ?
-
?

Organism

Organism UniProt Comment Textmining
Bacteroides thetaiotaomicron Q89ZI2
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography using nickel-affinity chromatography followed by gel filtration Bacteroides thetaiotaomicron

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O
-
Bacteroides thetaiotaomicron 4-methylumbelliferone + N-acetyl-beta-D-glucosamine
-
?
4-methylumbelliferyl N-difluoroacetyl-beta-D-glucosaminide + H2O
-
Bacteroides thetaiotaomicron 4-methylumbelliferone + N-difluoroacetyl-beta-D-glucosamine
-
?
4-methylumbelliferyl N-fluoroacetyl-beta-D-glucosaminide + H2O
-
Bacteroides thetaiotaomicron 4-methylumbelliferone + N-fluoroacetyl-beta-D-glucosamine
-
?
4-methylumbelliferyl N-trifluoroacetyl-beta-D-glucosaminide + H2O
-
Bacteroides thetaiotaomicron 4-methylumbelliferone + N-trifluoroacetyl-beta-D-glucosamine
-
?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
Bacteroides thetaiotaomicron 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
?
4-nitrophenyl N-acetyl-beta-D-thioglucosaminide + H2O
-
Bacteroides thetaiotaomicron 4-nitrophenol + N-acetyl-beta-D-thioglucosamine
-
?
additional information enzyme cleaves 4-nitrophenyl N-acetyl-beta-D-glucosaminide from modified proteins and also cleaves 4-nitrophenyl N-acetyl-beta-D-thioglucosaminide Bacteroides thetaiotaomicron ?
-
?

Subunits

Subunits Comment Organism
dimer
-
Bacteroides thetaiotaomicron

Synonyms

Synonyms Comment Organism
BtGH84
-
Bacteroides thetaiotaomicron
O-GlcNAcase
-
Bacteroides thetaiotaomicron

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bacteroides thetaiotaomicron

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
bell-shaped pH-activity profile Bacteroides thetaiotaomicron