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Literature summary for 3.2.1.169 extracted from

  • Wells, L.; Gao, Y.; Mahoney, J.A.; Vosseller, K.; Chen, C.; Rosen, A.; Hart, G.W.
    Dynamic O-glycosylation of nuclear and cytosolic proteins: further characterization of the nucleocytoplasmic beta-N-acetylglucosaminidase, O-GlcNAcase (2002), J. Biol. Chem., 277, 1755-1761.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpress human O-GlcNAcase as a fusion protein with thioredoxin in an Escherichia coli expression system Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Cricetulus griseus 5829
-
cytosol predominantly localized Homo sapiens 5829
-
nucleocytoplasm
-
Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
-
Chlorocebus aethiops

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Chlorocebus aethiops
-
-
-
Cricetulus griseus
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-affinity and ion-exchange chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Bos taurus
-
CHO-K1 cell
-
Cricetulus griseus
-
COS-7 cell african green monkey kidney Chlorocebus aethiops
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
Homo sapiens 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
?
Ala-Ser(O-GlcNAc)-Tyr + H2O is synthesized using standard N-(9-fluorenyl)methoxycarbonyl (FMOC) chemistry Homo sapiens Ala-Ser-Tyr + N-acetyl-beta-D-glucosamine
-
?

Subunits

Subunits Comment Organism
monomer
-
Homo sapiens

Synonyms

Synonyms Comment Organism
beta-N-acetylglucosaminidase
-
Cricetulus griseus
beta-N-acetylglucosaminidase
-
Homo sapiens
beta-N-acetylglucosaminidase
-
Bos taurus
beta-N-acetylglucosaminidase
-
Chlorocebus aethiops
O-GlcNAcase
-
Cricetulus griseus
O-GlcNAcase
-
Homo sapiens
O-GlcNAcase
-
Bos taurus
O-GlcNAcase
-
Chlorocebus aethiops

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide
-
Homo sapiens

General Information

General Information Comment Organism
metabolism caspase-3 efficiently cleaved O-GlcNAcase Chlorocebus aethiops