Cloned (Comment) | Organism |
---|---|
overexpress human O-GlcNAcase as a fusion protein with thioredoxin in an Escherichia coli expression system | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Cricetulus griseus | 5829 | - |
cytosol | predominantly localized | Homo sapiens | 5829 | - |
nucleocytoplasm | - |
Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
- |
Chlorocebus aethiops |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Chlorocebus aethiops | - |
- |
- |
Cricetulus griseus | - |
- |
- |
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel-affinity and ion-exchange chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
CHO-K1 cell | - |
Cricetulus griseus | - |
COS-7 cell | african green monkey kidney | Chlorocebus aethiops | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Homo sapiens | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
Ala-Ser(O-GlcNAc)-Tyr + H2O | is synthesized using standard N-(9-fluorenyl)methoxycarbonyl (FMOC) chemistry | Homo sapiens | Ala-Ser-Tyr + N-acetyl-beta-D-glucosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
beta-N-acetylglucosaminidase | - |
Cricetulus griseus |
beta-N-acetylglucosaminidase | - |
Homo sapiens |
beta-N-acetylglucosaminidase | - |
Bos taurus |
beta-N-acetylglucosaminidase | - |
Chlorocebus aethiops |
O-GlcNAcase | - |
Cricetulus griseus |
O-GlcNAcase | - |
Homo sapiens |
O-GlcNAcase | - |
Bos taurus |
O-GlcNAcase | - |
Chlorocebus aethiops |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | caspase-3 efficiently cleaved O-GlcNAcase | Chlorocebus aethiops |