Crystallization (Comment) | Organism |
---|---|
visualization of the reaction coordinate of O-GlcNAc hydrolases | Bacteroides thetaiotaomicron |
Protein Variants | Comment | Organism |
---|---|---|
D242N | mutant is active on the substrate 4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside, with an efficiency 320fold times lower than that of wild-type enzyme. D242 activates the 2-acetamido group for nucleophilic attack | Bacteroides thetaiotaomicron |
D243N | variant in which the general acid/base is impaired. D243 plays the role of general acid to aid departure of the leaving group. Crystals of the D242N variant grown at pH 8.5 with the substrate 4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside, an intact trapped oxazoline intermediate is observed | Bacteroides thetaiotaomicron |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside | D242N mutant, at a pH value of 8.5 the apparent Km is 12fold reduced (1 mM to 0.08 mM) | Bacteroides thetaiotaomicron | |
1 | - |
4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside | D242N mutant, at a pH value of 8.5 the apparent Km is 12fold reduced (1 mM to 0.08 mM) | Bacteroides thetaiotaomicron |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bacteroides thetaiotaomicron | when a substrate contains a modest leaving group as well as a poor nucleophile, the catalytic efficiency of the enzyme is synergistically impaired | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides thetaiotaomicron | Q89ZI2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-acetamido-2-deoxy-5-fluoro-beta-D-glucopyranosyl fluoride + H2O | a substrate contains a modest leaving group as well as a poor nucleophile, the catalytic efficiency of the enzyme is synergistically impaired. Testing a wide range of such substrates and obtained co-crystals of BtGH84, to test if under the right conditions, such a substrate might be trapped in the active site unhydrolyzed | Bacteroides thetaiotaomicron | fluoride + N-acetyl-beta-D-glucosaminide | - |
? | |
3,4-difluorophenyl 2-deoxy-2-difluoroacetamido-beta-D-glucopyranoside + H2O | a substrate contains a modest leaving group as well as a poor nucleophile, the catalytic efficiency of the enzyme is synergistically impaired. Testing a wide range of such substrates and obtained co-crystals of BtGH84, to test if under the right conditions, such a substrate might be trapped in the active site unhydrolyzed | Bacteroides thetaiotaomicron | 3,4-difluorophenol + 2-deoxy-2-difluoroacetamido-beta-D-glucopyranose | - |
? | |
4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O | substrate with a 4-methylumbelliferone leaving group. A substrate contains a modest leaving group as well as a poor nucleophile, the catalytic efficiency of the enzyme is synergistically impaired. Testing a wide range of such substrates and obtained co-crystals of BtGH84, to test if under the right conditions, such a substrate might be trapped in the active site unhydrolyzed | Bacteroides thetaiotaomicron | 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranose | - |
? | |
additional information | when a substrate contains a modest leaving group as well as a poor nucleophile, the catalytic efficiency of the enzyme is synergistically impaired | Bacteroides thetaiotaomicron | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BtGH84 | - |
Bacteroides thetaiotaomicron |
O-GlcNAc hydrolase | - |
Bacteroides thetaiotaomicron |
O-GlcNAcase | - |
Bacteroides thetaiotaomicron |
OGA | - |
Bacteroides thetaiotaomicron |