Cloned (Comment) | Organism |
---|---|
cloned and overexpressed in Escherichia coli | Clostridium perfringens |
Crystallization (Comment) | Organism |
---|---|
significant sequence homology to the human O-GlcNAcase N-terminus | Clostridium perfringens |
Protein Variants | Comment | Organism |
---|---|---|
D297N | rather inactive mutant | Clostridium perfringens |
D298N | rather inactive mutant | Clostridium perfringens |
D401A | rather inactive mutant | Clostridium perfringens |
N390A | as active as wild-type | Clostridium perfringens |
N396A | rather inactive mutant | Clostridium perfringens |
W490A | mutant with less activity | Clostridium perfringens |
Y335F | rather inactive mutant | Clostridium perfringens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ala-Cys(S-GlcNAc)-Ala | pseudosubstrate | Clostridium perfringens | |
O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate | PUGNAc, transition state mimic | Clostridium perfringens | |
streptozotocin | STZ, diabetogenic compound, acts as a suicide inhibitor | Clostridium perfringens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0029 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | wild-type | Clostridium perfringens | |
0.003 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | D297N mutant | Clostridium perfringens | |
0.0031 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | N390A mutant | Clostridium perfringens | |
0.004 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | D298N mutant | Clostridium perfringens | |
0.013 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | N396A mutant | Clostridium perfringens | |
0.015 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | D401A mutant | Clostridium perfringens | |
0.028 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | W490A mutant | Clostridium perfringens | |
0.041 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | Y335F mutant | Clostridium perfringens | |
0.121 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | - |
Clostridium perfringens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Clostridium perfringens | has O-GlcNAcase activity against O-GlcNAcylated human proteins, suggesting that the enzyme is a suitable model for further studies into the function of human O-GlcNAcase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium perfringens | Q0TR53 | - |
- |
Purification (Comment) | Organism |
---|---|
glutathione affinity chromatography | Clostridium perfringens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O | - |
Clostridium perfringens | 4-methylumbelliferone + N-acetyl-beta-D-glucosamine | - |
? | |
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Clostridium perfringens | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
additional information | has O-GlcNAcase activity against O-GlcNAcylated human proteins, suggesting that the enzyme is a suitable model for further studies into the function of human O-GlcNAcase | Clostridium perfringens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CpNagJ | - |
Clostridium perfringens |
NagJ | - |
Clostridium perfringens |
O-GlcNAcase | - |
Clostridium perfringens |
OGA | - |
Clostridium perfringens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | D298N mutant | Clostridium perfringens | |
0.0025 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | N396A mutant | Clostridium perfringens | |
0.0028 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | Y335F mutant | Clostridium perfringens | |
0.0044 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | D401A mutant | Clostridium perfringens | |
0.0198 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | D297N mutant | Clostridium perfringens | |
0.2 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | - |
Clostridium perfringens | |
4 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | W490A mutant | Clostridium perfringens | |
10.5 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | wild-type | Clostridium perfringens | |
11 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | N390A mutant | Clostridium perfringens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000054 | - |
O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate | - |
Clostridium perfringens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000086 | - |
wild-type | Clostridium perfringens | O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate | |
0.00001 | - |
N390A mutant | Clostridium perfringens | O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate | |
0.000024 | - |
W490A mutant | Clostridium perfringens | O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate | |
0.0049 | - |
- |
Clostridium perfringens | Ala-Cys(S-GlcNAc)-Ala | |
0.064 | - |
- |
Clostridium perfringens | streptozotocin |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0683 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | Y335F mutant | Clostridium perfringens | |
0.192 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | N396A mutant | Clostridium perfringens | |
0.293 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | D401A mutant | Clostridium perfringens | |
0.325 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | D298N mutant | Clostridium perfringens | |
6.6 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | D297N mutant | Clostridium perfringens | |
142.9 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | W490A mutant | Clostridium perfringens | |
3548 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | N390A mutant | Clostridium perfringens | |
3620 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | wild-type | Clostridium perfringens |