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Literature summary for 3.2.1.169 extracted from

  • Rao, F.V.; Dorfmueller, H.C.; Villa, F.; Allwood, M.; Eggleston, I.M.; van Aalten, D.M.
    Structural insights into the mechanism and inhibition of eukaryotic O-GlcNAc hydrolysis (2006), EMBO J., 25, 1569-1578.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
cloned and overexpressed in Escherichia coli Clostridium perfringens

Crystallization (Commentary)

Crystallization (Comment) Organism
significant sequence homology to the human O-GlcNAcase N-terminus Clostridium perfringens

Protein Variants

Protein Variants Comment Organism
D297N rather inactive mutant Clostridium perfringens
D298N rather inactive mutant Clostridium perfringens
D401A rather inactive mutant Clostridium perfringens
N390A as active as wild-type Clostridium perfringens
N396A rather inactive mutant Clostridium perfringens
W490A mutant with less activity Clostridium perfringens
Y335F rather inactive mutant Clostridium perfringens

Inhibitors

Inhibitors Comment Organism Structure
Ala-Cys(S-GlcNAc)-Ala pseudosubstrate Clostridium perfringens
O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate PUGNAc, transition state mimic Clostridium perfringens
streptozotocin STZ, diabetogenic compound, acts as a suicide inhibitor Clostridium perfringens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0029
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide wild-type Clostridium perfringens
0.003
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide D297N mutant Clostridium perfringens
0.0031
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide N390A mutant Clostridium perfringens
0.004
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide D298N mutant Clostridium perfringens
0.013
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide N396A mutant Clostridium perfringens
0.015
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide D401A mutant Clostridium perfringens
0.028
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide W490A mutant Clostridium perfringens
0.041
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide Y335F mutant Clostridium perfringens
0.121
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide
-
Clostridium perfringens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Clostridium perfringens has O-GlcNAcase activity against O-GlcNAcylated human proteins, suggesting that the enzyme is a suitable model for further studies into the function of human O-GlcNAcase ?
-
?

Organism

Organism UniProt Comment Textmining
Clostridium perfringens Q0TR53
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione affinity chromatography Clostridium perfringens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O
-
Clostridium perfringens 4-methylumbelliferone + N-acetyl-beta-D-glucosamine
-
?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
Clostridium perfringens 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
?
additional information has O-GlcNAcase activity against O-GlcNAcylated human proteins, suggesting that the enzyme is a suitable model for further studies into the function of human O-GlcNAcase Clostridium perfringens ?
-
?

Synonyms

Synonyms Comment Organism
CpNagJ
-
Clostridium perfringens
NagJ
-
Clostridium perfringens
O-GlcNAcase
-
Clostridium perfringens
OGA
-
Clostridium perfringens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0013
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide D298N mutant Clostridium perfringens
0.0025
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide N396A mutant Clostridium perfringens
0.0028
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide Y335F mutant Clostridium perfringens
0.0044
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide D401A mutant Clostridium perfringens
0.0198
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide D297N mutant Clostridium perfringens
0.2
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide
-
Clostridium perfringens
4
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide W490A mutant Clostridium perfringens
10.5
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide wild-type Clostridium perfringens
11
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide N390A mutant Clostridium perfringens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000054
-
O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate
-
Clostridium perfringens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000086
-
wild-type Clostridium perfringens O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate
0.00001
-
N390A mutant Clostridium perfringens O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate
0.000024
-
W490A mutant Clostridium perfringens O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate
0.0049
-
-
Clostridium perfringens Ala-Cys(S-GlcNAc)-Ala
0.064
-
-
Clostridium perfringens streptozotocin

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0683
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide Y335F mutant Clostridium perfringens
0.192
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide N396A mutant Clostridium perfringens
0.293
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide D401A mutant Clostridium perfringens
0.325
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide D298N mutant Clostridium perfringens
6.6
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide D297N mutant Clostridium perfringens
142.9
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide W490A mutant Clostridium perfringens
3548
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide N390A mutant Clostridium perfringens
3620
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide wild-type Clostridium perfringens