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Literature summary for 3.2.1.169 extracted from

  • Macauley, M.S.; Vocadlo, D.J.
    Enzymatic characterization and inhibition of the nuclear variant of human O-GlcNAcase (2009), Carbohydr. Res., 344, 1079-1084.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
(3aS,5R,6S,7R,7aR)-5-(hydroxymethyl)-2-methyl-5,6,7,7a-tetrahydro-3aH-pyrano[3,2-d][1,3]thiazole-6,7-diol NAG-thiazoline, inhibits also other functionally related enzymes Homo sapiens
(3aS,5R,6S,7R,7aR)-5-(hydroxymethyl)-2-propyl-5,6,7,7a-tetrahydro-3aH-pyrano[3,2-d][1,3]thiazole-6,7-diol NButGT, selective O-GlcNAcase inhibitor Homo sapiens
GlcNAc
-
Homo sapiens
N-[(2Z,3R,4R,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-2-[[(phenylcarbamoyl)oxy]imino]tetrahydro-2H-pyran-3-yl]acetamide PUGNAc, inhibits also other functionally related enzymes Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide OGA-FL Homo sapiens
0.32
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide OGA-NV Homo sapiens
1.5
-
N-acetyl-beta-D-glucosamine for OGA-FL Homo sapiens
3.6
-
N-acetyl-beta-D-glucosamine an inhibitor of OGA-NV Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens enzyme is responsible for removal of the O-GlcNAc post-translational modification, is being increasingly used to aid in discerning the roles played by this form of intracellular glycosylation ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
-
Homo sapiens 4-methylumbelliferol + N-acetyl-beta-D-glucosamine
-
?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
Homo sapiens 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
?
additional information enzyme is responsible for removal of the O-GlcNAc post-translational modification, is being increasingly used to aid in discerning the roles played by this form of intracellular glycosylation Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
O-GlcNAcase two forms of O-GlcNAcase have been studied, a full-length isoform that is better characterized, and a shorter nuclear-localized variant, arising from failure to splice out one intron Homo sapiens
OGA-FL full-length form of OGA Homo sapiens
OGA-NV nuclear variant Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
maximum activity Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0006
-
(3aS,5R,6S,7R,7aR)-5-(hydroxymethyl)-2-propyl-5,6,7,7a-tetrahydro-3aH-pyrano[3,2-d][1,3]thiazole-6,7-diol OGA-FL Homo sapiens
0.0015
-
(3aS,5R,6S,7R,7aR)-5-(hydroxymethyl)-2-propyl-5,6,7,7a-tetrahydro-3aH-pyrano[3,2-d][1,3]thiazole-6,7-diol OGA-NV Homo sapiens