Cloned (Comment) | Organism |
---|---|
O-GlcNAcase and its splice variant (v-O-GlcNAcase) expressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
v-O-GlcNAcase | variant OGlcNAcase, isoform lacking the C-terminal third of the full-length O-GlcNAcase | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate | PUGNAc | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0849 | - |
fluorescein di(N-acetyl-beta-D-glucosaminide) | O-GlcNAcase | Homo sapiens | |
0.43 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | - |
Homo sapiens | |
1.1 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | O-GlcNAcase | Homo sapiens | |
2.1 | - |
fluorescein di(N-acetyl-beta-D-glucosaminide) | splice variant (v-O-GlcNAcase) | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
on a nickel column via the His6-tag | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
HT-29 cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.78 | - |
fluorescein di(N-acetyl-beta-D-glucosaminide) as a substrate | Homo sapiens |
1.68 | - |
splice variant v-O-GlcNAcase, fluorescein di(N-acetyl-beta-D-glucosaminide) as a substrate | Homo sapiens |
2.035 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide as a substrate | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O | MUGlcNAc | Homo sapiens | 4-methylumbelliferone + N-acetyl-beta-D-glucosamine | - |
? | |
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Homo sapiens | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
fluorescein di(N-acetyl-beta-D-glucosaminide) + H2O | FDGlcNAc, fluorogenic substrate | Homo sapiens | fluorescein mono(N-acetyl-beta-D-glucosaminide) + N-acetyl-D-glucosamine | - |
? | |
additional information | fluorescein di-N-acetyl-beta-D-galactosaminide (FDGalNAc) is not cleaved by these enzymes, the O-GlcNAcase has substrate specificity toward O-GlcNAc but not O-GalNAc | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
O-GlcNAc specific beta-N-acetylglucosaminidase | - |
Homo sapiens |
O-GlcNAcase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
fluorescein di(N-acetyl-beta-D-glucosaminide) | - |
Homo sapiens | |
2.1 | - |
fluorescein di(N-acetyl-beta-D-glucosaminide) | splice variant (v-O-GlcNAcase) | Homo sapiens | |
3.4 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
fluorescein di(N-acetyl-beta-D-glucosaminide) | splice variant (v-O-GlcNAcase) | Homo sapiens | |
3.09 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | - |
Homo sapiens | |
15.3 | - |
fluorescein di(N-acetyl-beta-D-glucosaminide) | - |
Homo sapiens |