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Literature summary for 3.2.1.169 extracted from

  • Kim, E.J.; Kang, D.O.; Love, D.C.; Hanover, J.A.
    Enzymatic characterization of O-GlcNAcase isoforms using a fluorogenic GlcNAc substrate (2006), Carbohydr. Res., 341, 971-982.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
O-GlcNAcase and its splice variant (v-O-GlcNAcase) expressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
v-O-GlcNAcase variant OGlcNAcase, isoform lacking the C-terminal third of the full-length O-GlcNAcase Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate PUGNAc Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0849
-
fluorescein di(N-acetyl-beta-D-glucosaminide) O-GlcNAcase Homo sapiens
0.43
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
-
Homo sapiens
1.1
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide O-GlcNAcase Homo sapiens
2.1
-
fluorescein di(N-acetyl-beta-D-glucosaminide) splice variant (v-O-GlcNAcase) Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
on a nickel column via the His6-tag Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
Homo sapiens
-
HT-29 cell
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.78
-
fluorescein di(N-acetyl-beta-D-glucosaminide) as a substrate Homo sapiens
1.68
-
splice variant v-O-GlcNAcase, fluorescein di(N-acetyl-beta-D-glucosaminide) as a substrate Homo sapiens
2.035
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide as a substrate Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O MUGlcNAc Homo sapiens 4-methylumbelliferone + N-acetyl-beta-D-glucosamine
-
?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
Homo sapiens 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
?
fluorescein di(N-acetyl-beta-D-glucosaminide) + H2O FDGlcNAc, fluorogenic substrate Homo sapiens fluorescein mono(N-acetyl-beta-D-glucosaminide) + N-acetyl-D-glucosamine
-
?
additional information fluorescein di-N-acetyl-beta-D-galactosaminide (FDGalNAc) is not cleaved by these enzymes, the O-GlcNAcase has substrate specificity toward O-GlcNAc but not O-GalNAc Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
O-GlcNAc specific beta-N-acetylglucosaminidase
-
Homo sapiens
O-GlcNAcase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
fluorescein di(N-acetyl-beta-D-glucosaminide)
-
Homo sapiens
2.1
-
fluorescein di(N-acetyl-beta-D-glucosaminide) splice variant (v-O-GlcNAcase) Homo sapiens
3.4
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1
-
fluorescein di(N-acetyl-beta-D-glucosaminide) splice variant (v-O-GlcNAcase) Homo sapiens
3.09
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide
-
Homo sapiens
15.3
-
fluorescein di(N-acetyl-beta-D-glucosaminide)
-
Homo sapiens