Cloned (Comment) | Organism |
---|---|
construction of a pUC118-pTM hybrid expression vector containing the full length and splice variant mouse NCOAT | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
C166S | site-directed mutagenesis | Mus musculus |
C878S | site-directed mutagenesis, comparable level of O-GlcNAcase activity as wild-type enzyme | Mus musculus |
D174N | site-directed mutagenesis | Mus musculus |
D175A | site-directed mutagenesis | Mus musculus |
D177N | site-directed mutagenesis | Mus musculus |
DELTA250-345 | protein missing amino acids 250-345, due to the removal of exon 8 | Mus musculus |
DELTA250-398 | lacks amino acids 250-398 in the protein due to the specific excision of exons 8 and 9 | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HPDP-biotin | treatment with a sulfhydrylspecific biotinylation reagent, HPDP-biotin, to biotinylate any accessible free cysteine residue in the native enzyme. Treatment is able to inhibit enzymatic activity, suggesting that the cysteine residue playing a role in catalysis is modified | Mus musculus | |
N-ethylmaleimide | treatment is able to inhibit enzymatic activity, suggesting that the cysteine residue playing a role in catalysis is modified | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | D177N mutant | Mus musculus | |
0.46 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | C166S mutant | Mus musculus | |
0.49 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | wild-type | Mus musculus | |
0.97 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | D174N mutant | Mus musculus | |
1.4 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | D175A mutant | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9EQQ9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O | - |
Mus musculus | 4-methylumbelliferone + N-acetyl-beta-D-glucosamine | - |
? | |
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Mus musculus | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mNCOAT | - |
Mus musculus |
NCOAT | bifunctional nucleo-cytoplasmic protein with both O-GlcNAcase and histone acetyltransferase domains | Mus musculus |
O-GlcNAcase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | D177N mutant | Mus musculus | |
6.4 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | D175A mutant | Mus musculus | |
18.6 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | D174N mutant | Mus musculus | |
21.7 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | C166S mutant | Mus musculus | |
54.9 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | wild-type | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 5.5 | D174N mutant displayed a reduced catalytic efficiency at the wild type enzyme's optimal pH as well as in the basic pH range. It exhibits a marked shift in pH optimum to the pH 5-5.5 range | Mus musculus |
6.5 | 7 | optimum for the wild-type enzyme | Mus musculus |
7 | - |
assay at | Mus musculus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.6 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | D175A mutant | Mus musculus | |
8.8 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | D177N mutant | Mus musculus | |
19.2 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | D174N mutant | Mus musculus | |
47.2 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | C166S mutant | Mus musculus | |
112 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | wild-type | Mus musculus |