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Literature summary for 3.2.1.169 extracted from

  • Toleman, C.; Paterson, A.J.; Kudlow, J.E.
    Location and characterization of the O-GlcNAcase active site (2006), Biochim. Biophys. Acta, 1760, 829-839.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
construction of a pUC118-pTM hybrid expression vector containing the full length and splice variant mouse NCOAT Mus musculus

Protein Variants

Protein Variants Comment Organism
C166S site-directed mutagenesis Mus musculus
C878S site-directed mutagenesis, comparable level of O-GlcNAcase activity as wild-type enzyme Mus musculus
D174N site-directed mutagenesis Mus musculus
D175A site-directed mutagenesis Mus musculus
D177N site-directed mutagenesis Mus musculus
DELTA250-345 protein missing amino acids 250-345, due to the removal of exon 8 Mus musculus
DELTA250-398 lacks amino acids 250-398 in the protein due to the specific excision of exons 8 and 9 Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
HPDP-biotin treatment with a sulfhydrylspecific biotinylation reagent, HPDP-biotin, to biotinylate any accessible free cysteine residue in the native enzyme. Treatment is able to inhibit enzymatic activity, suggesting that the cysteine residue playing a role in catalysis is modified Mus musculus
N-ethylmaleimide treatment is able to inhibit enzymatic activity, suggesting that the cysteine residue playing a role in catalysis is modified Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.26
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide D177N mutant Mus musculus
0.46
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide C166S mutant Mus musculus
0.49
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide wild-type Mus musculus
0.97
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide D174N mutant Mus musculus
1.4
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide D175A mutant Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q9EQQ9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O
-
Mus musculus 4-methylumbelliferone + N-acetyl-beta-D-glucosamine
-
?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
Mus musculus 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
?

Synonyms

Synonyms Comment Organism
mNCOAT
-
Mus musculus
NCOAT bifunctional nucleo-cytoplasmic protein with both O-GlcNAcase and histone acetyltransferase domains Mus musculus
O-GlcNAcase
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide D177N mutant Mus musculus
6.4
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide D175A mutant Mus musculus
18.6
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide D174N mutant Mus musculus
21.7
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide C166S mutant Mus musculus
54.9
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide wild-type Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5 D174N mutant displayed a reduced catalytic efficiency at the wild type enzyme's optimal pH as well as in the basic pH range. It exhibits a marked shift in pH optimum to the pH 5-5.5 range Mus musculus
6.5 7 optimum for the wild-type enzyme Mus musculus
7
-
assay at Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.6
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide D175A mutant Mus musculus
8.8
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide D177N mutant Mus musculus
19.2
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide D174N mutant Mus musculus
47.2
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide C166S mutant Mus musculus
112
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide wild-type Mus musculus