Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.169 extracted from

  • Cetinbas, N.; Macauley, M.S.; Stubbs, K.A.; Drapala, R.; Vocadlo, D.J.
    Identification of Asp174 and Asp175 as the Key Catalytic Residues of Human O-GlcNAcase by Functional Analysis of Site-Directed Mutants (2006), Biochemistry, 45, 3835-3844.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
D174A generated mutant, reveals severely impaired catalytic activity across a broad range of substrates alongside a pH-activity profile consistent with deletion of a key catalytic residue Homo sapiens
D175A generated mutant, significant decrease in catalytic efficiency with substrates bearing poor leaving groups (up to 3000fold), while for substrates bearing good leading groups the difference is much smaller (7fold). Mutant enzyme cleaves thioglycosides with essentially the same catalytic efficiency as the wild-type enzyme Homo sapiens
DELTA1-350 deletion construct comprising amino acids 1-350 of human O-GlcNAcase Homo sapiens
DELTA351-916 deletion construct comprising amino acids 351-916 of human O-GlcNAcase Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide wild-type Homo sapiens
0.2
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide D175A mutant Homo sapiens
0.21
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide wild-type Homo sapiens
0.21
-
3,4-dinitrophenyl N-acetyl-beta-D-glucosaminide wild-type Homo sapiens
0.22
-
3,4-dinitrophenyl N-acetyl-beta-D-glucosaminide D175A mutant Homo sapiens
0.23
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide D175A mutant Homo sapiens
0.3
-
3-fluoro-4-nitrophenyl N-acetyl-beta-D-glucosaminide wild-type Homo sapiens
0.31
-
3-fluoro-4-nitrophenyl N-acetyl-beta-D-glucosaminide D175A mutant Homo sapiens
0.36
-
3,4-dinitrophenyl N-acetyl-beta-D-glucosaminide D174A mutant (pH 5.0) Homo sapiens
0.38
-
3-fluoro-4-nitrophenyl N-acetyl-beta-D-glucosaminide D174A mutant (pH 7.4) Homo sapiens
0.46
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide D174A mutant (pH 5.0) Homo sapiens
0.47
-
3-nitrophenyl N-acetyl-beta-D-glucosaminide wild-type Homo sapiens
0.56
-
4-chlorophenyl N-acetyl-beta-D-glucosaminide wild-type Homo sapiens
0.56
-
3,4-dinitrophenyl N-acetyl-beta-D-glucosaminide D174A mutant (pH 7.4) Homo sapiens
0.62
-
phenyl N-acetyl-beta-D-glucosaminide wild-type Homo sapiens
0.73
-
3-fluoro-4-nitrophenyl N-acetyl-beta-D-glucosaminide D174A mutant (pH 5.0) Homo sapiens
0.84
-
N-acetyl-4-aminophenyl N-acetyl-beta-D-glucosaminide wild-type Homo sapiens
0.95
-
3-nitrophenyl N-acetyl-beta-D-glucosaminide D175A mutant Homo sapiens
0.95
-
phenyl N-acetyl-beta-D-glucosaminide D175A mutant Homo sapiens
1.4
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide D174A mutant (pH 7.4) Homo sapiens
1.4
-
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide D174A mutant (pH 7.4) Homo sapiens
1.5
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens catalyzing the hydrolytic cleavage of beta-O-linked 2-acetamido-2-deoxy-D-glycopyranose (O-GlcNAc) from serine and threonine residues of posttranslationally modified proteins ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-affinity chromotography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dinitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
Homo sapiens 3,4-dinitrophenol + N-acetyl-beta-D-glucosamine
-
?
3-fluoro-4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
Homo sapiens 3-fluoro-4-nitrophenol + N-acetyl-beta-D-glucosamine
-
?
3-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
Homo sapiens 3-nitrophenol + N-acetyl-beta-D-glucosamine
-
?
4-chlorophenyl N-acetyl-beta-D-glucosaminide + H2O
-
Homo sapiens 4-chlorophenol + N-acetyl-beta-D-glucosamine
-
?
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O
-
Homo sapiens 4-methylumbelliferone + N-acetyl-beta-D-glucosamine
-
?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
Homo sapiens 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
?
additional information catalyzing the hydrolytic cleavage of beta-O-linked 2-acetamido-2-deoxy-D-glycopyranose (O-GlcNAc) from serine and threonine residues of posttranslationally modified proteins Homo sapiens ?
-
?
N-acetyl-4-aminophenyl N-acetyl-beta-D-glucosaminide + H2O
-
Homo sapiens N-acetyl-4-aminophenol + N-acetyl-beta-D-glucosamine
-
?
phenyl N-acetyl-beta-D-glucosaminide + H2O
-
Homo sapiens phenol + N-acetyl-beta-D-glucosamine
-
?

Synonyms

Synonyms Comment Organism
O-GlcNAcase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
measured pH-values range from 4.5 to 9.0, hydrolysis of 4-nitrophenyl N-acetyl-beta-D-glucosaminide resembles a bell-shaped curve with the maximal catalytic efficiency at a pH of 6.5 Homo sapiens
7.4
-
assay at Homo sapiens