Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C896W | site-specific mutagenesis, in the tryptic peptide LGCFEIAK (894-901). Site-specific mutagenesis of the C-terminal glycine and cysteine residues (G895A and C896W) present in the tryptic peptide LGCFEIAK (894901) causes little or no inhibition of O-GlcNAcase activity (72.3% of control and 129.9% of control, respectively) | Homo sapiens |
E130A | site-specific mutagenesis, in the tryptic peptide EYEIEFIYAISPGLDITFSNPK (128-149), retains almost full enzymatic activity (97.3% of control) | Homo sapiens |
F133V | site-specific mutagenesis, in the tryptic peptide EYEIEFIYAISPGLDITFSNPK (128-149), no O-GlcNAcase activity (3.3% of control) | Homo sapiens |
G895A | site-specific mutagenesis, in the tryptic peptide LGCFEIAK (894-901). Site-specific mutagenesis of the C-terminal glycine and cysteine residues (G895A and C896W) present in the tryptic peptide LGCFEIAK (894901) causes little or no inhibition of O-GlcNAcase activity (72.3% of control and 129.9% of control, respectively) | Homo sapiens |
S138I | site-specific mutagenesis, in the tryptic peptide EYEIEFIYAISPGLDITFSNPK (128-149), exhibits greatly reduced activity (15.4% of control) | Homo sapiens |
Y129F | site-specific mutagenesis, in the tryptic peptide EYEIEFIYAISPGLDITFSNPK (128-149), retains almost full enzymatic activity (90.5% of control) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | methylnitrosourea (MNU), the nitric oxide-containing portion of streptozotocin: no significant inhibition observed | Homo sapiens | |
O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenylcarbamate | PUGNAc | Homo sapiens | |
streptozotocin | STZ, significantly dose-dependently inhibited recombinant O-GlcNAcase | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
recombinant O-GlcNAcase, Coomassie blue staining following one-dimensional gel electrophoresis and Western blotting | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | enzyme removes N-acetyl-beta-D-glucosaminide from proteins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Homo sapiens | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
additional information | enzyme removes N-acetyl-beta-D-glucosaminide from proteins | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
O-GlcNAc-selective N-acetyl-beta-D-glucosaminidase | - |
Homo sapiens |
O-GlcNAcase | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |