Cloned (Comment) | Organism |
---|---|
- |
Clostridium perfringens |
expressed in Escherichia coli BL21 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
V331C | mutation of Val331 to cysteine results in a mutant enzyme with unaltered steady-state kinetics compared with wild-type CpOGA | Clostridium perfringens |
W490A | mutation of Trp490 to alanine results in a mutant enzyme that shows a 30fold reduction in Km when assayed with the pseudosubstrate 4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide | Clostridium perfringens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GlcNAcstatin A | GlcNAcstatin A carries a carboxymethyl group, GlcNAcstatins are the most potent human O-GlcNAcase inhibitors, inhibiting the enzyme in the sub-nanomolar to nanomolar range | Homo sapiens | |
GlcNAcstatin B | GlcNAcstatin B carries a phenylethyl moiety. GlcNAcstatins are the most potent human O-GlcNAcase inhibitors, inhibiting the enzyme in the sub-nanomolar to nanomolar range | Homo sapiens | |
GlcNAcstatin C | GlcNAcstatin C carriesa phenylethyl moiety. GlcNAcstatins are the most potent human OGA inhibitors, inhibiting the enzyme in the sub-nanomolar to nanomolar range | Clostridium perfringens | |
GlcNAcstatin C | GlcNAcstatin C carriesa phenylethyl moiety. GlcNAcstatins are the most potent human O-GlcNAcase inhibitors, inhibiting the enzyme in the sub-nanomolar to nanomolar range | Homo sapiens | |
GlcNAcstatin D | GlcNAcstatin D carries a phenylethyl moiety. GlcNAcstatins are the most potent human O-GlcNAcase inhibitors, inhibiting the enzyme in the sub-nanomolar to nanomolar range | Homo sapiens | |
GlcNAcstatin E | GlcNAcstatin E carries a phenylethyl moiety. GlcNAcstatins are the most potent human O-GlcNAcase inhibitors, inhibiting the enzyme in the sub-nanomolar to nanomolar range | Homo sapiens | |
O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino N-phenylcarbamate | PUGNAc | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0029 | - |
4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide | wild-type | Clostridium perfringens | |
0.0068 | - |
4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide | V331C mutant | Clostridium perfringens | |
0.1 | - |
4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide | W490A mutant | Clostridium perfringens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | enzyme that removes N-acetyl-beta-D-glucosamine from O-GlcNAcylated proteins | ? | - |
? | |
additional information | Clostridium perfringens | enzyme that removes O-GlcNAc from O-GlcNAcylated proteins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium perfringens | Q0TR53 | - |
- |
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Clostridium perfringens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293 cell | - |
Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
HT-1080 cell | - |
Homo sapiens | - |
SH-SY5Y cell | - |
Homo sapiens | - |
U2-OS cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O | fluorogenic substrate, 4MU-NAG | Homo sapiens | 4-methylumbelliferone + N-acetyl-beta-D-glucosamine | - |
? | |
4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide + H2O | fluorogenic substrate, 4MU-NAG | Clostridium perfringens | 4-methylumbelliferone + N-acetyl-beta-D-glucosamine | - |
? | |
additional information | enzyme that removes N-acetyl-beta-D-glucosamine from O-GlcNAcylated proteins | Homo sapiens | ? | - |
? | |
additional information | enzyme that removes O-GlcNAc from O-GlcNAcylated proteins | Clostridium perfringens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CpOGA | - |
Clostridium perfringens |
hOGA | - |
Homo sapiens |
O-GlcNAcase | - |
Homo sapiens |
O-GlcNAcase | - |
Clostridium perfringens |
OGA | - |
Homo sapiens |
OGA | - |
Clostridium perfringens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.5 | - |
4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide | wild-type | Clostridium perfringens | |
17.3 | - |
4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide | V331C mutant | Clostridium perfringens | |
65 | - |
4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide | W490A mutant | Clostridium perfringens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000042 | - |
GlcNAcstatin B | - |
Homo sapiens | |
0.00000074 | - |
GlcNAcstatin D | - |
Homo sapiens | |
0.0000043 | - |
GlcNAcstatin A | - |
Homo sapiens | |
0.0000044 | - |
GlcNAcstatin C | - |
Homo sapiens | |
0.0000046 | - |
GlcNAcstatin C | wild-type | Clostridium perfringens | |
0.000035 | - |
O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino N-phenylcarbamate | - |
Homo sapiens | |
0.000074 | - |
GlcNAcstatin C | W490A mutant | Clostridium perfringens | |
0.0000981 | - |
GlcNAcstatin C | V331C mutant | Clostridium perfringens | |
0.0085 | - |
GlcNAcstatin E | - |
Homo sapiens |