Any feedback?
Literature summary for 3.2.1.167 extracted from
- The relationship between endogenous beta-glucuronidase activity and biologically active flavones-aglycone contents in hairy roots of Baikal skullcap (2018), Chem. Biodivers., 15, e1700409 .
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| baicalin + H2O | Scutellaria baicalensis | - |
baicalein + D-glucuronate | - |
? |  |
| additional information | Scutellaria baicalensis | domination of the glucuronide wogonoside is a characteristic feature of the hairy roots culture, while glucuronide baicalin is predominant in intact plant roots and also in the hairy roots. Alteration in contents of the main flavones in hairy roots culture of the Baikal skullcap during culturing, overview | ? | - |
? | |
| wogonoside + H2O | Scutellaria baicalensis | - |
wogonin + D-glucuronate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Scutellaria baicalensis | Q9LRC8 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hairy root | - |
Scutellaria baicalensis | - |
| additional information | relationship between contents of principal flavones in hairy roots of Scutellaria baicalensis with the activity of the beta-glucuronidase (sGUS) enzyme during a culturing cycle, overview. The highest contents of aglycones, baicalin and wogonin is observed at the growth days 8, 14, and 71 and reach 45, 41, and 62% (based on the total weight of hairy roots of the Baikal skullcap), correspondingly. Their accumulation is accompanied by increase of the sGUS activity. The enzyme activity is characterized by significant and reasonable correlation only with the wogonin contents | Scutellaria baicalensis | - |
| root | - |
Scutellaria baicalensis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-beta-D-glucuronide + H2O | - |
Scutellaria baicalensis | 4-methylumbelliferol + D-glucuronate | - |
? | |
| baicalin + H2O | - |
Scutellaria baicalensis | baicalein + D-glucuronate | - |
? | |
| additional information | domination of the glucuronide wogonoside is a characteristic feature of the hairy roots culture, while glucuronide baicalin is predominant in intact plant roots and also in the hairy roots. Alteration in contents of the main flavones in hairy roots culture of the Baikal skullcap during culturing, overview | Scutellaria baicalensis | ? | - |
? | |
| wogonoside + H2O | - |
Scutellaria baicalensis | wogonin + D-glucuronate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| baicalinase | - |
Scutellaria baicalensis |
| beta-glucuronidase | - |
Scutellaria baicalensis |
| GUS | - |
Scutellaria baicalensis |
| sGUS | - |
Scutellaria baicalensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Scutellaria baicalensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Scutellaria baicalensis |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Scutellaria baicalensis | increase in the glucuronidase activity due to mechanical stress (e.g. dissecting roots) | up |
| Scutellaria baicalensis | increase in the glucuronidase activity during the first weeks of cultivation followed by mechanical stress (e.g. dissecting roots) | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the central precursor metabolite of wogonin and baicalein and their glycosylated forms is chrysin | Scutellaria baicalensis |
| physiological function | relationship between contents of principal flavones in hairy roots of Scutellaria baicalensis with the activity of the beta-glucuronidase (sGUS) enzyme during a culturing cycle. The enzyme activity is characterized by significant and reasonable correlation only with the wogonin contents. The endogenous beta-glucuronidase (sGUS), that catalyzes the cleavage of the glucuronic acid residue from the glycosylated forms of phenolic compounds | Scutellaria baicalensis |
| physiological function | the beta-glucuronidase enzyme is involved in the formation of the more active aglycone flavones in the mdicinal plant. The enzyme activity is characterized by significant correlation only with the wogonin contents, whereas the correlation for the baicalin is insignificant | Scutellaria baicalensis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
