Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta 2 (DE3) | Levilactobacillus brevis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.154 | - |
4-nitrophenyl beta-D-glucuronide | native enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
0.156 | - |
4-nitrophenyl beta-D-glucuronide | recombinant His-tagged enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
1.5 | 2 | Baicalin | native enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
1.74 | - |
Baicalin | recombinant His-tagged enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
2.02 | - |
wogonoside | recombinant His-tagged enzyme, pH 5.0, 37°C | Levilactobacillus brevis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, native enzyme, SDS-PAGE | Levilactobacillus brevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
baicalin + H2O | Levilactobacillus brevis | the enzyme prefers baicalin rather than estrone 3-(beta-D-glucuronide), one of the human endogenous steroid hormones | baicalein + D-glucuronate | - |
? | |
baicalin + H2O | Levilactobacillus brevis FERM BP-4693 | the enzyme prefers baicalin rather than estrone 3-(beta-D-glucuronide), one of the human endogenous steroid hormones | baicalein + D-glucuronate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Levilactobacillus brevis | - |
subsp. coagulans | - |
Levilactobacillus brevis FERM BP-4693 | - |
subsp. coagulans | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) by nickel affinity chromatography and dialysis, native enzyme 1800fold from cell-free extract by repeated anion exchange chromatography and gel filtration, followed by dialysis | Levilactobacillus brevis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.57 | - |
purified native enzyme, pH 5.0, 37°C, substrate baicalin | Levilactobacillus brevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucuronide + H2O | - |
Levilactobacillus brevis | 4-nitrophenol + D-glucuronate | - |
? | |
4-nitrophenyl beta-D-glucuronide + H2O | - |
Levilactobacillus brevis FERM BP-4693 | 4-nitrophenol + D-glucuronate | - |
? | |
baicalin + H2O | the enzyme prefers baicalin rather than estrone 3-(beta-D-glucuronide), one of the human endogenous steroid hormones | Levilactobacillus brevis | baicalein + D-glucuronate | - |
? | |
baicalin + H2O | the enzyme is strictly glycon-specific but not aglycon-specific | Levilactobacillus brevis | baicalein + D-glucuronate | - |
? | |
baicalin + H2O | the enzyme prefers baicalin rather than estrone 3-(beta-D-glucuronide), one of the human endogenous steroid hormones | Levilactobacillus brevis FERM BP-4693 | baicalein + D-glucuronate | - |
? | |
baicalin + H2O | the enzyme is strictly glycon-specific but not aglycon-specific | Levilactobacillus brevis FERM BP-4693 | baicalein + D-glucuronate | - |
? | |
estrone 3-(beta-D-glucuronide) + H2O | - |
Levilactobacillus brevis | estrone + D-glucuronate | - |
? | |
estrone 3-(beta-D-glucuronide) + H2O | - |
Levilactobacillus brevis FERM BP-4693 | estrone + D-glucuronate | - |
? | |
additional information | substrate specificity, the enzyme is inactive with other 4-nitrophenyl glycosides, overview | Levilactobacillus brevis | ? | - |
? | |
additional information | substrate specificity, the enzyme is inactive with other 4-nitrophenyl glycosides, overview | Levilactobacillus brevis FERM BP-4693 | ? | - |
? | |
wogonoside + H2O | - |
Levilactobacillus brevis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, native enzyme, SDS-PAGE | Levilactobacillus brevis |
Synonyms | Comment | Organism |
---|---|---|
LcGUS30 | - |
Levilactobacillus brevis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Levilactobacillus brevis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | activity range, profile overview | Levilactobacillus brevis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | purified enzyme, stable at | Levilactobacillus brevis |
70 | - |
purified enzyme, inactivation | Levilactobacillus brevis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.37 | - |
wogonoside | recombinant His-tagged enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
18.5 | - |
Baicalin | recombinant His-tagged enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
21.3 | - |
Baicalin | native enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
31.8 | - |
4-nitrophenyl beta-D-glucuronide | recombinant His-tagged enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
53.7 | - |
4-nitrophenyl beta-D-glucuronide | native enzyme, pH 5.0, 37°C | Levilactobacillus brevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Levilactobacillus brevis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 7 | activity range, profile overview | Levilactobacillus brevis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
purified enzyme, inactivation | Levilactobacillus brevis |
3.5 | 9 | purified enzyme, stable at | Levilactobacillus brevis |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycoside hydrolase family 30, GH30, subfamily 3 | Levilactobacillus brevis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.58 | - |
estrone 3-(beta-D-glucuronide) | recombinant His-tagged enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
3.15 | - |
wogonoside | recombinant His-tagged enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
10.6 | - |
Baicalin | recombinant His-tagged enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
14 | - |
Baicalin | native enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
204 | - |
4-nitrophenyl beta-D-glucuronide | recombinant His-tagged enzyme, pH 5.0, 37°C | Levilactobacillus brevis | |
348 | - |
4-nitrophenyl beta-D-glucuronide | native enzyme, pH 5.0, 37°C | Levilactobacillus brevis |