General Stability | Organism |
---|---|
the calcium alginate-encapsulated GUS retains up to 88% of its free-form activity with an encapsulation efficiency of 77%. Conversion of baicalin by free and calcium alginate-encapsulated GUS results in the baicalein productivities of 80% and 65%, respectively. The calcium alginate-encapsulated GUS shows no appreciable loss in activity after four repeated cycles, and 90% of its initial activity remains after 26-day storage at 4°C | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the free enzyme activity decreases rapidly and is even totally lost when the Ca2+ concentration is higher than 5 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
5,6,7-trihydroxyflavone-7-beta-D-glucuronide | free enzyme, at pH 7.0 and 37°C | Escherichia coli | |
0.36 | - |
5,6,7-trihydroxyflavone-7-beta-D-glucuronide | calcium alginate-encapsulated enzyme, at pH 7.0 and 37°C | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Escherichia coli | 5764 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
290000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Storage Stability | Organism |
---|---|
4°C, 30 mM Tris-HCl pH 7.0, 11 days, 26% loss of activity | Escherichia coli |
4°C, 30 mM Tris-HCl pH 7.0, 5 days, 19% loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6,7-trihydroxyflavone-7-beta-D-glucuronide + H2O | - |
Escherichia coli | 5,6,7-trihydroxyflavone + D-glucuronate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-glucuronidase | - |
Escherichia coli |
EC 3.2.1.31 | - |
Escherichia coli |
GUS | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | the free enzyme shows about 45% relative activity at 30°C, about 65% relative activity at 40°C, about 80% relative activity at 50°C, 100% activity at 60°C, and about 75% relative activity at 70°C (at pH 7.0, reaction time 40 min) | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | the free enzyme shows no activity at pH 4.0, about 85% relative activity at pH 5.5 and pH 6.5, 100% activity at pH 7.0, about 60% relative activity at pH 7.5, and no activity at pH 8.0 (at 37°C, reaction time 60 min) | Escherichia coli |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli | isoelectric focusing | - |
4.8 |