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Literature summary for 3.2.1.167 extracted from
- Purification and characterization of baicalin-b-D-glucuronidase hydrolyzing baicalin to baicalein from fresh roots of Scutellaria viscidula Bge (2005), Process Biochem., 40, 1911-1915.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | inhibits 29% at 100 mM | Scutellaria viscidula |  |
| Fe2+ | inhibits 29% at 1 mM, 71% at 100 mM | Scutellaria viscidula | |
| Zn2+ | inhibits 7% at 100 mM | Scutellaria viscidula | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates 7% at 100 mM | Scutellaria viscidula | |
| Mg2+ | activates 26% at 100 mM | Scutellaria viscidula | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58400 | - |
x * 58400, SDS-PAGE | Scutellaria viscidula |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| baicalin + H2O | Scutellaria viscidula | - |
baicalein + beta-D-glucuronic acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Scutellaria viscidula | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 7.2fold from roots, by ammonium sulfate fractionation and anion exchange chromatography to homogeneity | Scutellaria viscidula |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | fresh | Scutellaria viscidula | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Scutellaria viscidula |
| 9.37 | - |
purified enzyme, substrate 4-nitrophenyl beta-D-glucuronide | Scutellaria viscidula |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucuronide + H2O | - |
Scutellaria viscidula | 4-nitrophenol + beta-D-glucuronic acid | - |
? | |
| 4-phenolphthaleinyl beta-D-glucuronide + H2O | - |
Scutellaria viscidula | 4-phenolphthalein + beta-D-glucuronic acid | - |
? | |
| baicalin + H2O | - |
Scutellaria viscidula | baicalein + beta-D-glucuronic acid | - |
? | |
| baicalin + H2O | best substrate | Scutellaria viscidula | baicalein + beta-D-glucuronic acid | - |
? | |
| additional information | no activity with 4-nitrophenyl beta-D-glucoside | Scutellaria viscidula | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 58400, SDS-PAGE | Scutellaria viscidula |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| baicalin-beta-D-glucuronidase | - |
Scutellaria viscidula |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Scutellaria viscidula |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | - |
Scutellaria viscidula |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
30 min, stable up to | Scutellaria viscidula |
| 80 | - |
inactivation | Scutellaria viscidula |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Scutellaria viscidula |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | - |
Scutellaria viscidula |
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