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Literature summary for 3.2.1.167 extracted from

  • Ikegami, F.; Matsunae, K.; Hisamitsu, M.; Kurihara, T.; Yamamoto, T.; Murakoshi, I.
    Purification and properties of a plant beta-D-glucuronidase from Scutellaria root (1995), Biol. Pharm. Bull., 18, 1531-1534.
    View publication on PubMed
Search for more literature for 3.2.1.167

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 0.3 mM, 20-25% inhibition Scutellaria baicalensis
Ca2+ 0.3 mM, about 15% inhibition Scutellaria baicalensis
Cu2+ 0.3 mM, 20-25% inhibition Scutellaria baicalensis
D-glucose 10 mM, 15% inhibition Scutellaria baicalensis
D-glucuronate 10 mM, 15% inhibition Scutellaria baicalensis
additional information 2-mercaptoethanol has no influence on activity up to 10 mM Scutellaria baicalensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
 5,6,7-trihydroxyflavone 7-O-beta-D-glucoronate
-
 Scutellaria baicalensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 4 * 55000, SDS-PAGE Scutellaria baicalensis
200000 220000 gel filtration Scutellaria baicalensis

Organism

Organism UniProt Comment Textmining
Scutellaria baicalensis
-
 Georg
-
Scutellaria baicalensis Georg
-
 Georg
-

Purification (Commentary)

Purification (Comment) Organism
-
 Scutellaria baicalensis

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Scutellaria baicalensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
114
-
-
 Scutellaria baicalensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate + H2O i.e. baicalin Scutellaria baicalensis 5,6,7-trihydroxyflavone + D-glucuronate i.e. baicalein ?
5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate + H2O i.e. baicalin Scutellaria baicalensis Georg 5,6,7-trihydroxyflavone + D-glucuronate i.e. baicalein ?
5,7-dihydroxy-8-methoxyflavone-7-O-beta-D-glucuronate + H2O i.e. wogonin 7-O-beta-D-glucuronide, i.e. 5-hydroxy-8-methoxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid Scutellaria baicalensis 5,7-dihydroxy-8-methoxyflavone + D-glucuronate
-
 ?
5,7-dihydroxy-8-methoxyflavone-7-O-beta-D-glucuronate + H2O i.e. wogonin 7-O-beta-D-glucuronide, i.e. 5-hydroxy-8-methoxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid Scutellaria baicalensis Georg 5,7-dihydroxy-8-methoxyflavone + D-glucuronate
-
 ?
additional information the enzyme is not able to cleave the bond between the two glucuronic acid moieties in glycyrrhizin (glycyrrhetic acid 3-O-D-diglucuronide), the enzyme is not capable of hydrolyzing glucosides, luteolin-7-glucoside and apigenin-7-O-glucoside Scutellaria baicalensis ?
-
 ?
additional information the enzyme is not able to cleave the bond between the two glucuronic acid moieties in glycyrrhizin (glycyrrhetic acid 3-O-D-diglucuronide), the enzyme is not capable of hydrolyzing glucosides, luteolin-7-glucoside and apigenin-7-O-glucoside Scutellaria baicalensis Georg ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 55000, SDS-PAGE Scutellaria baicalensis

Synonyms

Synonyms Comment Organism
baicalinase
-
 Scutellaria baicalensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 50
-
 Scutellaria baicalensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7
-
 in 50 mM Na-citrate buffer Scutellaria baicalensis

pI Value

Organism Comment pI Value Maximum pI Value
Scutellaria baicalensis chromatofocusing
-
 5.4