Any feedback?
Literature summary for 3.2.1.165 extracted from
- The structure of an archaeal beta-glucosaminidase provides insight into glycoside hydrolase evolution (2017), J. Biol. Chem., 292, 4996-5006 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta (DE3)pLysS cells | Pyrococcus horikoshii |
| expressed in Escherichia coli Rosetta (DE3)pLysS cells | Thermococcus kodakarensis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| in complex with D-glucosamine, sitting drop vapor diffusion method, using in 100 mM Tris-HCl (pH 8.5) and 20% (w/v) polyethylene glycol 1000 | Pyrococcus horikoshii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D178N | inactive | Thermococcus kodakarensis |
| D180N | inactive | Pyrococcus horikoshii |
| E179Q | the mutant shows 3% of wild type activity | Thermococcus kodakarensis |
| E181Q | the mutant shows 10% of wild type activity | Pyrococcus horikoshii |
| E181Q | the mutant shows 15% of wild type activity | Pyrococcus horikoshii |
| E306Q | the mutant shows 40% of wild type activity | Thermococcus kodakarensis |
| E347Q | inactive | Thermococcus kodakarensis |
| E349Q | inactive | Pyrococcus horikoshii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus horikoshii | - |
- |
- |
| Thermococcus kodakarensis | Q76HN4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography, HiTrap Q column chromatography and Superdex 200 gel filtration | Pyrococcus horikoshii |
| Ni-NTA column chromatography, HiTrap Q column chromatography and Superdex 200 gel filtration | Thermococcus kodakarensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitobiose + H2O | - |
Pyrococcus horikoshii | 2 D-glucosamine | - |
? |  |
| chitobiose + H2O | - |
Thermococcus kodakarensis | 2 D-glucosamine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exo-beta-D-glucosaminidase | - |
Pyrococcus horikoshii |
| exo-beta-D-glucosaminidase | - |
Thermococcus kodakarensis |
| GlmA | - |
Pyrococcus horikoshii |
| GlmA | - |
Thermococcus kodakarensis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
