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Literature summary for 3.2.1.165 extracted from

  • Ike, M.; Ogasawara, W.; Okada, H.; Morikawa, Y.
    The essential acidic amino acid residues for catalytic activity of an exo-beta-D-glucosaminidase from Trichoderma reesei (2008), J. Mol. Catal. B, 55, 55-60 .
No PubMed abstract available
Search for more literature for 3.2.1.165

Protein Variants

Protein Variants Comment Organism
D464A specific acitivity is 0.44% compared to the activity of the wild-type enzyme Trichoderma reesei
D464E no activity Trichoderma reesei
D464E/E539D no activity Trichoderma reesei
D464N specific acitivity is 1.9% compared to the activity of the wild-type enzyme Trichoderma reesei
D513N specific acitivity is 72% compared to the activity of the wild-type enzyme Trichoderma reesei
D655N specific acitivity is 103% compared to the activity of the wild-type enzyme Trichoderma reesei
E409Q specific acitivity is 77% compared to the activity of the wild-type enzyme Trichoderma reesei
E415Q specific acitivity is 53% compared to the activity of the wild-type enzyme Trichoderma reesei
E470Q specific acitivity is 99% compared to the activity of the wild-type enzyme Trichoderma reesei
E539A no activity Trichoderma reesei
E539D no activity Trichoderma reesei
E539Q no activity Trichoderma reesei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 chitosan Km-value (mg/ml): 0.104 (wild-type enzyme), 0.221 (mutant enzyme E409Q), 0.140 (mutant enzyme E415Q), 0.352 (mutant enzyme D513N), pH 4.0 Trichoderma reesei

Organism

Organism UniProt Comment Textmining
Trichoderma reesei Q4R1C4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trichoderma reesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O chitosan 10B Trichoderma reesei D-glucosamine + ?
-
 ?

Synonyms

Synonyms Comment Organism
Gls93
-
 Trichoderma reesei