Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95000 | - |
- |
Purpureocillium lilacinum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Purpureocillium lilacinum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by cation-exchange chromatography from chitosan-grown cells | Purpureocillium lilacinum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
hydrolysis of chitosan or chitosan oligosaccharides to remove successive D-glucosamine residues from the non-reducing termini | two-step mechanism and transglycosylation of the retaining glycoside hydrolase forming a glycosyl-enzyme intermediate | Purpureocillium lilacinum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Purpureocillium lilacinum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitobiose + H2O | (GlcN)6 is sequentially cleaved into GlcN5,GlcN4, GlcN3, GlcN2, and GlcN, glucosamine is the final product | Purpureocillium lilacinum | glucosamine + glucosamine | - |
? | |
chitohexaose + H2O | (GlcN)6 is sequentially cleaved into GlcN5,GlcN4, GlcN3, GlcN2, and GlcN, glucosamine is the final product | Purpureocillium lilacinum | chitopentaose + glucosamine | - |
? | |
chitopentaose + H2O | (GlcN)6 is sequentially cleaved into GlcN5,GlcN4, GlcN3, GlcN2, and GlcN, glucosamine is the final product | Purpureocillium lilacinum | chitotetraose + glucosamine | - |
? | |
chitotetraose + H2O | (GlcN)6 is sequentially cleaved into GlcN5,GlcN4, GlcN3, GlcN2, and GlcN, glucosamine is the final product | Purpureocillium lilacinum | chitotriose + glucosamine | - |
? | |
chitotriose + H2O | (GlcN)6 is sequentially cleaved into GlcN5,GlcN4, GlcN3, GlcN2, and GlcN, glucosamine is the final product | Purpureocillium lilacinum | chitobiose + D-glucosamine | - |
? | |
additional information | the enzyme cleaves the beta-1->4-glycosidic bonds between GlcN-GlcN and GlcN-GlcNAc but not between GlcNAc-GlcN or GlcNAc-GlcNAc, cleavage of GlcN-GlcNAc, a partially acetylated chitooligomer mixture, overview. The purified enzyme displays high activity toward chitosan and (GlcN)6. No significant activity with N-carboxymethyl chitosan, or chitin, also synthetic p-nitrophenyl-beta-D-glucosaminide is not hydrolyzed. Chitosan with higher deacetylation degrees is hydrolyzed more efficiently than chitosan with lower deacetylation values. Substrate specifiicty analysis using massspectrometry. Paecilomyces exo-beta-D-GlcNase can accelerate the hydrolysis of p-nitrophenyl-beta-D-N-acetylglucosaminide, p-nitrophenyl-beta-D-N,N'-diacetylchitobioside, and p-nitrophenyl-beta-D-N-acetylgalactosaminide to release the corresponding sugar | Purpureocillium lilacinum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
exo-beta--D-GlcNase | - |
Purpureocillium lilacinum |
exo-beta-D-glucosaminidase | - |
Purpureocillium lilacinum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Purpureocillium lilacinum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Purpureocillium lilacinum |
Organism | Comment | Expression |
---|---|---|
Purpureocillium lilacinum | the enzyme is induced by chitosan | up |