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Literature summary for 3.2.1.165 extracted from

  • Li, S.; Wang, C.; Xia, W.
    Expression, purification, and characterization of exo-beta-D-glucosaminidase of Aspergillus sp. CJ22-326 from Escherichia coli (2009), Carbohydr. Res., 344, 1046-1049.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the His-tagged enzyme His-csx is expressed in Escherichia coli BL21(DE3) cells Aspergillus sp.

Organism

Organism UniProt Comment Textmining
Aspergillus sp. C3U4R9
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-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and ultrafiltration Aspergillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitosan + H2O 100% activity with 95% deacetylated chitosan, 88% activity with 83% deacetylated chitosan, 80% activity with 70% deacetylated chitosan, and 68% activity with 60% deacetylated chitosan Aspergillus sp. D-glucosamine + ?
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?
additional information no hydrolysis of colloid chitin and carboxymethylcellulose Aspergillus sp. ?
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?

Synonyms

Synonyms Comment Organism
csx recombinant enzyme Aspergillus sp.
exo-beta-D-glucosaminidase
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Aspergillus sp.