Literature summary for 3.2.1.165 extracted from

Sakamoto, Y.; Ike, M.; Tanaka, N.; Suzuki, Y.; Ogasawara, W.; Okada, H.; Nonaka, T.; Morikawa, Y.; Nakamura, K.T.
Crystallization and preliminary X-ray crystallographic studies of an exo-beta-D-glucosaminidase from Trichoderma reesei (2010), Acta Crystallogr. Sect. F, 66, 309-312.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Pichia pastoris	Trichoderma reesei

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 14% (w/v) PEG 8000 and 0.2 M ammonium acetate in 0.1 M cacodylate buffer pH 7.0 for enzyme form Gls93-F1 or 20% (w/v) PEG 4000 and 0.2 M sodium isothiocyanate for enzyme form Gls93-F2	Trichoderma reesei

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Trichoderma reesei	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
100000	-	form Gls93-F2, SDS-PAGE	Trichoderma reesei
105000	-	form Gls93-F1, SDS-PAGE	Trichoderma reesei

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
chitosan + H2O	Trichoderma reesei	-	D-glucosamine + ?	-	?

Organism

Organism	UniProt	Comment	Textmining
Trichoderma reesei	Q4R1C4	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, Q-Sepharose column chromatography, phenyl Sepharose column chromatography, and Sephacryl S-100 gel filtration	Trichoderma reesei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
chitosan + H2O	-	Trichoderma reesei	D-glucosamine + ?	-	?

Synonyms

Synonyms	Comment	Organism
exo-beta-D-glucosaminidase	-	Trichoderma reesei
GlcNase	-	Trichoderma reesei
Gls93	-	Trichoderma reesei

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Release 2023.1 (January 2023)