BRENDA - Enzyme Database
show all sequences of 3.2.1.165

Purification and characterization of the exo-beta -D-glucosaminidase from Aspergillus flavus IAM2044

Ji, J.H.; Yang, J.S.; Hur, J.W.; J. Microbiol. Biotechnol. 13, 269-275 (2003)
No PubMed abstract available

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
gel filtrration
Aspergillus flavus
Organism
Organism
UniProt
Commentary
Textmining
Aspergillus flavus
-
-
-
Aspergillus flavus IAM2044
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Aspergillus flavus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture filtrate
-
Aspergillus flavus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1167
-
-
Aspergillus flavus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
chitobiose + H2O
-
681334
Aspergillus flavus
D-glucosamine
-
-
-
?
chitobiose + H2O
-
681334
Aspergillus flavus IAM2044
D-glucosamine
-
-
-
?
chitohexaose + H2O
chitohexaose is hydrolyzed to chitopentaose and D-glucosamine at the initial stage of the reaction. The final product is D-glucosamine
681334
Aspergillus flavus
D-glucosamine
-
-
-
?
chitohexaose + H2O
chitohexaose is hydrolyzed to chitopentaose and D-glucosamine at the initial stage of the reaction. The final product is D-glucosamine
681334
Aspergillus flavus IAM2044
D-glucosamine
-
-
-
?
chitopentaose + H2O
-
681334
Aspergillus flavus
D-glucosamine
-
-
-
?
chitopentaose + H2O
-
681334
Aspergillus flavus IAM2044
D-glucosamine
-
-
-
?
chitosan + H2O
-
681334
Aspergillus flavus
D-glucosamine
-
-
-
?
chitosan + H2O
-
681334
Aspergillus flavus IAM2044
D-glucosamine
-
-
-
?
chitotetraose + H2O
-
681334
Aspergillus flavus
D-glucosamine
-
-
-
?
chitotriose + H2O
-
681334
Aspergillus flavus
D-glucosamine
-
-
-
?
glycol chitosan + H2O
-
681334
Aspergillus flavus
?
-
-
-
?
glycol chitosan + H2O
-
681334
Aspergillus flavus IAM2044
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 45000, SDS-PAGE
Aspergillus flavus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Aspergillus flavus
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
60
30°C: about 70% of maximal activity, 60°C: about 45% of maximal activity
Aspergillus flavus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
15 min, stable below
Aspergillus flavus
60
-
15 min, about 80% loss of activity
Aspergillus flavus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Aspergillus flavus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
6
pH 4.0: about 55% of maximal activity, pH 6.0: about 60% of maximal activity
Aspergillus flavus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
3.5
7
37°C, 15 min, more than 50% of initial activity remains
Aspergillus flavus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
gel filtrration
Aspergillus flavus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Aspergillus flavus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture filtrate
-
Aspergillus flavus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1167
-
-
Aspergillus flavus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
chitobiose + H2O
-
681334
Aspergillus flavus
D-glucosamine
-
-
-
?
chitobiose + H2O
-
681334
Aspergillus flavus IAM2044
D-glucosamine
-
-
-
?
chitohexaose + H2O
chitohexaose is hydrolyzed to chitopentaose and D-glucosamine at the initial stage of the reaction. The final product is D-glucosamine
681334
Aspergillus flavus
D-glucosamine
-
-
-
?
chitohexaose + H2O
chitohexaose is hydrolyzed to chitopentaose and D-glucosamine at the initial stage of the reaction. The final product is D-glucosamine
681334
Aspergillus flavus IAM2044
D-glucosamine
-
-
-
?
chitopentaose + H2O
-
681334
Aspergillus flavus
D-glucosamine
-
-
-
?
chitopentaose + H2O
-
681334
Aspergillus flavus IAM2044
D-glucosamine
-
-
-
?
chitosan + H2O
-
681334
Aspergillus flavus
D-glucosamine
-
-
-
?
chitosan + H2O
-
681334
Aspergillus flavus IAM2044
D-glucosamine
-
-
-
?
chitotetraose + H2O
-
681334
Aspergillus flavus
D-glucosamine
-
-
-
?
chitotriose + H2O
-
681334
Aspergillus flavus
D-glucosamine
-
-
-
?
glycol chitosan + H2O
-
681334
Aspergillus flavus
?
-
-
-
?
glycol chitosan + H2O
-
681334
Aspergillus flavus IAM2044
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 45000, SDS-PAGE
Aspergillus flavus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Aspergillus flavus
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
30
60
30°C: about 70% of maximal activity, 60°C: about 45% of maximal activity
Aspergillus flavus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
15 min, stable below
Aspergillus flavus
60
-
15 min, about 80% loss of activity
Aspergillus flavus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Aspergillus flavus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
6
pH 4.0: about 55% of maximal activity, pH 6.0: about 60% of maximal activity
Aspergillus flavus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
3.5
7
37°C, 15 min, more than 50% of initial activity remains
Aspergillus flavus
Other publictions for EC 3.2.1.165
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
752333
Aktuganov
Purification and characteriza ...
Penicillium sp. IB-37-2A
World J. Microbiol. Biotechnol.
35
18
2019
4
-
-
-
-
-
8
-
-
2
-
-
-
5
-
-
1
-
-
-
2
-
7
1
3
1
-
1
-
-
1
1
-
-
1
-
4
-
-
-
-
-
-
-
8
-
-
-
2
-
-
-
-
-
1
-
-
2
-
7
1
1
-
1
-
-
1
1
1
-
-
-
-
-
-
750784
Kim
Production of chitinase from ...
Chryseobacterium indologenes, Comamonas koreensis, Chryseobacterium indologenes HANYOO, Comamonas koreensis HANWOO
Int. J. Biol. Macromol.
112
1115-1121
2018
-
2
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
2
2
-
-
-
2
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
751103
Mine
The structure of an archaeal ...
Pyrococcus horikoshii, Thermococcus kodakarensis
J. Biol. Chem.
292
4996-5006
2017
-
-
2
1
8
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
8
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
749436
Sinha
Enzymatic production of gluco ...
Aspergillus fumigatus, Aspergillus fumigatus IIT-004
3 Biotech
6
13
2016
2
-
-
-
-
1
17
-
-
1
-
2
-
4
-
-
1
-
-
1
2
1
17
1
2
3
-
1
1
2
-
-
-
-
1
-
2
-
-
-
-
-
1
-
17
-
-
-
1
-
2
-
-
-
1
-
1
2
1
17
1
3
-
1
1
2
-
-
1
-
-
-
-
-
-
749852
Honda
The crystal structure of an i ...
Photobacterium profundum, Photobacterium profundum SS9
Biochem. J.
473
463-472
2016
-
-
1
1
5
-
-
5
-
-
-
-
-
5
-
-
1
-
-
-
-
-
4
-
2
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
1
5
-
-
-
-
5
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
5
-
-
-
-
-
-
-
-
8
8
751306
Wu
Heterologous expression and c ...
Aspergillus oryzae, Aspergillus oryzae FL402
J. Microbiol. Biotechnol.
26
347-355
2016
-
-
1
-
1
-
11
2
-
1
-
-
-
2
-
1
-
-
-
-
-
-
2
2
2
1
1
1
2
1
1
-
-
-
-
-
-
-
1
-
-
1
-
-
11
-
2
-
1
-
-
-
-
1
-
-
-
-
-
2
2
1
1
1
2
1
1
-
-
-
-
-
-
2
2
729832
Nidheesh
-
Enzymatic degradation of chito ...
Penicillium decumbens, Penicillium decumbens CFRNT15
Int. Biodeter. Biodegrad.
97
97-106
2015
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
1
-
-
4
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
729529
Chao
Catalytic function of a newly ...
Purpureocillium lilacinum
Carbohydr. Polym.
93
615-621
2013
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
1
-
1
-
-
6
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
6
-
1
-
-
-
1
-
-
-
1
-
-
1
-
-
715133
Honda
Elucidation of exo-beta-D-gluc ...
Photobacterium profundum, Photobacterium profundum SS9
Glycobiology
21
503-511
2011
-
-
1
-
-
-
-
9
-
-
-
-
-
10
-
-
1
-
-
-
2
-
17
-
2
1
-
-
9
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
1
-
-
2
-
17
-
1
-
-
9
1
1
-
-
-
-
-
-
10
10
701549
Sakamoto
Crystallization and preliminar ...
Trichoderma reesei
Acta Crystallogr. Sect. F
66
309-312
2010
-
-
1
1
-
-
-
-
1
-
2
1
-
6
-
-
1
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
2
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699562
van Bueren
The structural basis of substr ...
Amycolatopsis orientalis
J. Mol. Biol.
385
131-139
2009
-
-
1
1
2
-
-
-
-
-
1
2
-
3
-
-
1
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
2
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
703053
Li
Expression, purification, and ...
Aspergillus sp.
Carbohydr. Res.
344
1046-1049
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
8
-
-
1
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
706044
Pluvinage
Inhibition of the exo-beta-D-g ...
Amycolatopsis orientalis
Org. Biomol. Chem.
7
4169-4172
2009
-
-
-
-
-
-
3
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
3
2
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699070
Dennhart
Oligosaccharide hydrolysis by ...
Amycolatopsis orientalis
J. Biotechnol.
134
253-260
2008
-
-
1
-
-
-
-
-
-
-
-
1
-
4
-
-
1
-
-
-
1
-
2
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
748421
Ike
-
The essential acidic amino ac ...
Trichoderma reesei
J. Mol. Catal. B
55
55-60
2008
-
-
-
-
12
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
680406
Fukamizo
-
Structure and function of exo- ...
Amycolatopsis orientalis
J. Appl. Glycosci.
54
133-138
2007
-
-
1
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677725
Ike
Cloning and heterologous expre ...
Trichoderma reesei, Trichoderma reesei PC-3-7
Appl. Microbiol. Biotechnol.
72
687-695
2006
-
-
1
-
-
-
-
-
-
-
2
-
-
7
-
1
1
-
-
1
-
-
2
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
1
-
1
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
678026
Cote
Two exo-beta-D-glucosaminidase ...
Amycolatopsis orientalis
Biochem. J.
394
675-686
2006
-
-
-
-
-
-
-
1
-
-
1
-
-
6
-
-
1
-
-
2
-
-
8
1
1
1
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
2
-
-
8
1
1
-
-
1
1
-
-
1
-
-
-
-
-
-
680031
Fukamizo
Exo-beta-D-glucosaminidase fro ...
Amycolatopsis orientalis
Glycobiology
16
1064-1072
2006
-
1
-
-
5
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
682639
Jung
Purification and characterizat ...
Aspergillus fumigatus, Aspergillus fumigatus S-26
Protein Expr. Purif.
45
125-131
2006
-
-
-
-
-
-
8
1
1
-
-
-
-
5
-
-
1
-
-
1
1
-
13
-
-
1
1
1
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
8
-
1
1
-
-
-
-
-
-
1
-
1
1
-
13
-
1
1
1
1
1
-
1
-
-
-
-
-
-
-
680443
Tanaka
Characterization of an exo-bet ...
Thermococcus kodakarensis
J. Bacteriol.
185
5175-5181
2003
-
-
1
-
-
-
-
5
-
-
2
-
-
9
-
-
1
-
-
-
-
-
6
1
1
1
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
5
-
-
2
-
-
-
-
1
-
-
-
-
6
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
681334
Ji
-
Purification and characterizat ...
Aspergillus flavus, Aspergillus flavus IAM2044
J. Microbiol. Biotechnol.
13
269-275
2003
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
1
1
-
12
1
-
1
1
2
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
1
-
12
1
1
1
2
-
1
1
1
-
-
-
-
-
-
-
646767
Zhang
Purification and characterizat ...
Aspergillus oryzae, Aspergillus oryzae IAM2660
Biosci. Biotechnol. Biochem.
64
1896-1902
2000
-
-
-
-
-
-
-
-
-
-
1
-
-
8
-
-
1
-
-
1
1
1
6
1
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
1
1
6
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
678869
Matsumura
-
One-step preparation of alkyl ...
Talaromyces funiculosus, Talaromyces funiculosus KY616
Biotechnol. Lett.
21
451-456
1999
-
-
-
-
-
-
-
3
1
-
1
-
-
2
-
-
1
-
-
1
1
-
11
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
1
-
1
-
-
-
-
1
-
1
1
-
11
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
677651
Nogawa
Purification and characterizat ...
Trichoderma reesei, Trichoderma reesei PC-3-7
Appl. Environ. Microbiol.
64
890-895
1998
-
-
-
-
-
-
-
-
-
-
1
-
-
9
-
-
1
-
-
1
1
-
8
1
-
1
-
1
-
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
1
-
8
1
1
-
1
-
1
1
2
-
-
-
-
-
-
-
677420
Uchida
-
Purification and properties of ...
Penicillium sp., Penicillium sp. AF9-P-112
Adv. Chitin Sci.
2
244-249
1997
-
1
-
-
-
-
-
-
-
-
2
-
-
2
-
-
1
-
-
1
1
-
12
1
1
1
-
1
-
1
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
1
1
-
12
1
1
-
1
-
1
-
1
1
-
-
-
-
-
-
680593
Nanjo
Purification and characterizat ...
Amycolatopsis orientalis
J. Biol. Chem.
265
10088-10094
1990
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
1
-
-
11
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-