Literature summary for 3.2.1.165 extracted from

  • Matsumura, S.; Yao, E.; Toshima, K.
    One-step preparation of alkyl beta-D-glucosaminide by the transglycosylation of chitosan and alcohol using purified exo-beta-D-glucosaminidase (1999), Biotechnol. Lett., 21, 451-456.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
chitobiose
-
Talaromyces funiculosus
0.15
-
chitotetraose
-
Talaromyces funiculosus
0.96
-
chitotriose
-
Talaromyces funiculosus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Talaromyces funiculosus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
108000
-
x * 108000, SDS-PAGE Talaromyces funiculosus

Organism

Organism UniProt Comment Textmining
Talaromyces funiculosus
-
-
-
Talaromyces funiculosus KY616
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Talaromyces funiculosus

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
Talaromyces funiculosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
41.2
-
-
Talaromyces funiculosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme also catalyzes transglycosylation reactions Talaromyces funiculosus ?
-
?
additional information the enzyme also catalyzes transglycosylation reactions Talaromyces funiculosus KY616 ?
-
?
chitobiose + H2O
-
Talaromyces funiculosus D-glucosamine
-
?
chitobiose + H2O
-
Talaromyces funiculosus KY616 D-glucosamine
-
?
chitosan + H2O only D-glucosamine is produced after prolonged hydrolysis Talaromyces funiculosus D-glucosamine + ?
-
?
chitosan + H2O only D-glucosamine is produced after prolonged hydrolysis Talaromyces funiculosus KY616 D-glucosamine + ?
-
?
chitotetraose + H2O
-
Talaromyces funiculosus D-glucosamine
-
?
chitotetraose + H2O
-
Talaromyces funiculosus KY616 D-glucosamine
-
?
chitotriitol + H2O
-
Talaromyces funiculosus D-glucosamine
-
?
chitotriitol + H2O
-
Talaromyces funiculosus KY616 D-glucosamine
-
?
chitotriose + H2O
-
Talaromyces funiculosus chitobiose + D-glucosamine
-
?

Subunits

Subunits Comment Organism
? x * 108000, SDS-PAGE Talaromyces funiculosus

Synonyms

Synonyms Comment Organism
exo-beta-D-GlcNase
-
Talaromyces funiculosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 70 substrate: chitosan Talaromyces funiculosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
substrate: chitosan Talaromyces funiculosus