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Literature summary for 3.2.1.165 extracted from

  • Nogawa, M.; Takahashi, H.; Kashiwagi, A.; Ohshima, K.; Okada, H.; Morikawa, Y.
    Purification and characterization of exo-beta-D-glucosaminidase from a cellulolytic fungus, Trichoderma reesei PC-3-7 (1998), Appl. Environ. Microbiol., 64, 890-895.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.165

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
92000
-
 gel filtration Trichoderma reesei

Organism

Organism UniProt Comment Textmining
Trichoderma reesei
-
-
-
Trichoderma reesei PC-3-7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trichoderma reesei

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Trichoderma reesei
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27.3
-
-
 Trichoderma reesei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitohexaose + H2O cleaved to chitopentaose and glusosamine in the initial stage of the reaction Trichoderma reesei D-glucosamine
-
 ?
chitohexaose + H2O cleaved to chitopentaose and glusosamine in the initial stage of the reaction Trichoderma reesei PC-3-7 D-glucosamine
-
 ?
chitopentaose + H2O
-
 Trichoderma reesei D-glucosamine
-
 ?
chitopentaose + H2O
-
 Trichoderma reesei PC-3-7 D-glucosamine
-
 ?
chitosan + H2O the exo-beta-D-glucosaminidase cleaves the glycosidic link of either GlcN-beta(1,4)-GlcN or GlcN-beta(1,4)-GlcNAc Trichoderma reesei ?
-
 ?
chitosan + H2O the exo-beta-D-glucosaminidase cleaves the glycosidic link of either GlcN-beta(1,4)-GlcN or GlcN-beta(1,4)-GlcNAc Trichoderma reesei PC-3-7 ?
-
 ?
p-nitrophenyl-beta-D-N-acetylglucosaminide + H2O
-
 Trichoderma reesei p-nitrophenol + beta-D-N-acetylglucosamine
-
 ?
p-nitrophenyl-beta-D-N-acetylglucosaminide + H2O
-
 Trichoderma reesei PC-3-7 p-nitrophenol + beta-D-N-acetylglucosamine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 92000, SDS-PAGE Trichoderma reesei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Trichoderma reesei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 pH 4.0, 1 h, stable Trichoderma reesei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Trichoderma reesei

pH Range

pH Minimum pH Maximum Comment Organism
3.5 4.5 pH 3.5: about 60% of maximal activity, pH 4.5: about 50% of maximal activity Trichoderma reesei

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
 1 h, 37°C, about 70% loss of activity Trichoderma reesei
6 9 1 h, 37°C, stable Trichoderma reesei