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Literature summary for 3.2.1.164 extracted from

  • Takata, R.; Tokita, K.; Mori, S.; Shimoda, R.; Harada, N.; Ichinose, H.; Kaneko, S.; Igarashi, K.; Samejima, M.; Tsumuraya, Y.; Kotake, T.
    Degradation of carbohydrate moieties of arabinogalactan-proteins by glycoside hydrolases from Neurospora crassa (2010), Carbohydr. Res., 345, 2516-2522.
    View publication on PubMed
Search for more literature for 3.2.1.164

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51264
-
 x * 51264, calculated from amino acid sequence Neurospora crassa
54000
-
 x * 54000, SDS-PAGE Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa Q7S2S4
-
-

Purification (Commentary)

Purification (Comment) Organism
CM-32 column chromatography, DEAE-Sepharose column chromatography and Sephadex G-100 gel filtration Neurospora crassa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
192
-
 culture medium, using beta-(1->3)(1->6)-galactan as substrate, at pH 5.0 and 37°C Neurospora crassa
292
-
 after fold purification, using beta-(1->3)(1->6)-galactan as substrate, at pH 5.0 and 37°C Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arabinogalactan-protein + H2O the enzyme prefers alpha-L arabinofuranosidase-treated arabinogalactan-proteins from radish roots (151% activity) and radish leaves (87% activity) compared to native ones from radish roots (36% activity) and leaves (28% activity) Neurospora crassa beta-GlcA-(1->6)-Gal + 4-methyl-beta-GlcA-(1->6)-Gal
-
 ?
beta-(1->3)(1->6)-galactan + H2O 100% activity Neurospora crassa ?
-
 ?
beta-(1->6)-galactotriose + H2O
-
 Neurospora crassa beta-(1->6)-galactobiose + galactose
-
 ?
additional information the enzyme fails to act on gum arabic, beta-(1->6)-galactobiose beza-(1->4)-galactan from lupin, beta-(1->3)(1->4)-glucan from barley, beta-(1->3)(1->6)-glucan from Laminaria digitata, beta-(1->6)-glucan from Umbilicaria papullosa, carboxymethyl-curdlan (beta-(1->3)-glucan), carboxymethyl-cellulose (beta-(1->4)-glucan), galactomannan from guar, galactomannan from locust bean, beta-(1->4)-xylan from birchwood, arabino-beta-(1->4)-xylan from wheat flour, arabinan (alpha-(1->3)(1->5)-arabinan), and debranched arabinan (alpha-(1->5)-arabinan) Neurospora crassa ?
-
 ?

Subunits

Subunits Comment Organism
? x * 54000, SDS-PAGE Neurospora crassa
? x * 51264, calculated from amino acid sequence Neurospora crassa

Synonyms

Synonyms Comment Organism
6GAL
-
 Neurospora crassa
endo-beta-(1->6)-galactanase
-
 Neurospora crassa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Neurospora crassa